@inbook{d390f89f457a41bf9e6422d061b4ad1a,
title = "Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER)",
abstract = "Virtually all methods for reading the sequence of bases in DNA rely on the ability to denature double-stranded DNA into single strands and then use Watson-Crick base-pairing rules to hybridize the strands with high specificity to another DNA primer or probe. However, nature frequently uses an alternative method, reading the sequence information directly from double-stranded DNA using sequence-specific DNA-binding proteins. Here we describe methods for the construction and testing of sequence probes based on engineered zinc finger DNA-binding proteins. Background is reduced using split-reporter molecules, and signal is amplified using enzymatic reporters. The resulting sequence-enabled reassembly (SEER) probes can be configured to detect DNA sequence (genetic) or DNA methylation (epigenetic) information.",
keywords = "DNA sequence, Double-stranded DNA, beta-lactamase, diagnostics, engineered zinc finger proteins, fluorescent detection, green florescent protein, luciferase, methylation",
author = "Porter, {Jason R.} and Lockwood, {Sarah H.} and Segal, {David J.} and Indraneel Ghosh",
year = "2010",
doi = "10.1007/978-1-60761-753-2_23",
language = "English (US)",
isbn = "9781607617525",
series = "Methods in Molecular Biology",
pages = "365--382",
editor = "Joel Mackay and David Segal",
booktitle = "Engineered Zinc Finger Proteins",
}