Abstract
This study describes the isolation of second-site suppressors which correct for the defects associated with cold-sensitive (cs) prohead accessory proteins of bacteriophage ΦX174. Five phenotypically different suppressors were isolated. Three of these suppressors confer novel temperature-sensitive (ts) phenotypes. They were unable to complement a ts mutation in gene F which encodes the major coat protein of the phage. All five suppressor mutations confer nucleotide changes in the gene F DNA sequence. These changes define four amino acid sites in the gene F protein. Three suppressor mutations placed into an otherwise wild-type background display a cold resistant phenotype in liquid culture infections when compared to a wild-type ΦX174 control.
Original language | English (US) |
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Pages (from-to) | 663-671 |
Number of pages | 9 |
Journal | Genetics |
Volume | 128 |
Issue number | 4 |
State | Published - 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics