Sample preparation method for metal(loid) contaminant quantitation in rodent hair collected in Yuma County, Arizona

Jonathan Credo, Amy Chandos, Camilla Checinski, Frank A. von Hippel, Jani C. Ingram

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Yuma County, Arizona, is a large agricultural hub of the USA located in the southwestern corner of Arizona on the USA-Mexico border. Year-round use of agrichemicals at a massive scale along with the influx of aquatic contaminants in the Colorado River led to significant levels of environmental pollution and hence exposure risks for people and wildlife. Although hair is a recognized biomarker for metal exposure, there is no universal hair preparation protocol. This study evaluated two digestion methods for metal quantitation using inductively coupled plasma-mass spectrometry (ICP-MS) and three methods for mercury quantitation using cold vapor-atomic absorption spectroscopy (CV-AAS), both employing certified reference materials. The “overnight” and “heating” digestion methods were suitable for ICP-MS, while only the heating method was suitable for CV-AAS. These validated methods will be useful for a variety of human and wildlife assessments of toxic metal(loid) exposure.

Original languageEnglish (US)
Article number522
JournalEnvironmental Monitoring and Assessment
Issue number8
StatePublished - Aug 2021


  • Agrichemical exposure
  • CV-AAS
  • ICP-MS
  • Rodent animal model
  • Sample preparation

ASJC Scopus subject areas

  • General Environmental Science
  • Pollution
  • Management, Monitoring, Policy and Law

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