Rotavirus-Specific CD5+ B Cells in Young Children Exhibit a Distinct Antibody Repertoire Compared with CD5- B Cells

Jörn Hendrik Weitkamp, Bonnie J. LaFleur, James E. Crowe

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Antiviral antibody responses in infants are limited in quality. One reason for this finding could be that the majority of B cells in infants are CD5+ cells, a subset of B cells that is thought to contain cells expressing polyreactive, low-affinity B cell receptors. We analyzed the rotavirus (RV)-specific antibody heavy chain variable region (VH) repertoire in CD5+ and CD5- B cells of four RV-infected children between 10 and 19 months of age. We found that the RV-specific B cell repertoire in CD5+ cells was VH3 family biased, in contrast to the VH1/VH4 dominance seen in CD5- B cells. The immunodominant RV-specific gene segment in CD5- B cells was VH1-46, which is the dominant segment used in RV-specific peripheral blood B cells from infants and adults. In contrast, the immunodominant gene segment was VH3-23 in RV-specific CD5+ B cells, which is the dominant gene segment in randomly selected B cells. Both RV-specific CD5+ and RV-specific CD5- B cells from all children studied demonstrated very low frequencies of somatic mutations. In conclusion, CD5+ B cells in infants responding to RV use an antibody gene repertoire that differs from the virus-specific repertoire of CD5- B cells, and both CD5+ and CD5- RV-specific B cells exhibit a low frequency of somatic mutations.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalHuman Immunology
Issue number1-2
StatePublished - Jan 2006
Externally publishedYes


  • B lymphocytes
  • CD5 antigen
  • human
  • Rotavirus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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