Root organogenesis from single cells released from the root cap of Medicago sp.

Martha C. Hawes; Laura Y. Smith; Martha Stephenson

doi:10.1007/BF00157595

Root organogenesis from single cells released from the root cap of Medicago sp.

Martha C. Hawes, Laura Y. Smith, Martha Stephenson

Environmental Science, Research

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.

Original language	English (US)
Pages (from-to)	303-308
Number of pages	6
Journal	Plant Cell, Tissue and Organ Culture
Volume	27
Issue number	3
DOIs	https://doi.org/10.1007/BF00157595
State	Published - Dec 1991

Keywords

Alfalfa
alfalfa tissue culture
root border cells
root cap
sloughed root cap cells

ASJC Scopus subject areas

Horticulture

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10.1007/BF00157595

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title = "Root organogenesis from single cells released from the root cap of Medicago sp.",

abstract = "Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.",

keywords = "Alfalfa, alfalfa tissue culture, root border cells, root cap, sloughed root cap cells",

author = "Hawes, {Martha C.} and Smith, {Laura Y.} and Martha Stephenson",

year = "1991",

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pages = "303--308",

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AU - Hawes, Martha C.

AU - Smith, Laura Y.

AU - Stephenson, Martha

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N2 - Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.

AB - Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.

KW - Alfalfa

KW - alfalfa tissue culture

KW - root border cells

KW - root cap

KW - sloughed root cap cells

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ER -

Root organogenesis from single cells released from the root cap of Medicago sp.

Abstract

Keywords

ASJC Scopus subject areas

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