Abstract
Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.
Original language | English (US) |
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Pages (from-to) | 1037-1044 |
Number of pages | 8 |
Journal | Insect Biochemistry and Molecular Biology |
Volume | 32 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2002 |
Keywords
- Aedes aegypti
- Mosquito
- R1
- R2
- Ribonucleotide reductase
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Insect Science