Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin

Zhenxing Liu; Chongyu Liao; Luming Zou; Minghui Jin; Yinxue Shan; Yudong Quan; Hui Yao; Lei Zhang; Peng Wang; Zhuangzhuang Liu; Na Wang; Anjing Li; Kaiyu Liu; Bruce E. Tabashnik; David G. Heckel; Kongming Wu; Yutao Xiao

doi:10.1371/journal.pbio.3002704

Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin

Zhenxing Liu, Chongyu Liao, Luming Zou, Minghui Jin, Yinxue Shan, Yudong Quan, Hui Yao, Lei Zhang, Peng Wang, Zhuangzhuang Liu, Na Wang, Anjing Li, Kaiyu Liu, Bruce E. Tabashnik, David G. Heckel, Kongming Wu, Yutao Xiao

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Abstract

The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has been produced by transgenic crops to counter pest resistance to the widely used crystalline (Cry) insecticidal proteins from Bt. To proactively manage pest resistance, there is an urgent need to better understand the genetic basis of resistance to Vip3Aa, which has been largely unknown. We discovered that retrotransposon-mediated alternative splicing of a midgut-specific chitin synthase gene was associated with 5,560-fold resistance to Vip3Aa in a laboratory-selected strain of the fall armyworm, a globally important crop pest. The same mutation in this gene was also detected in a field population. Knockout of this gene via CRISPR/ Cas9 caused high levels of resistance to Vip3Aa in fall armyworm and 2 other lepidopteran pests. The insights provided by these results could help to advance monitoring and management of pest resistance to Vip3Aa.

Original language	English (US)
Article number	e3002704
Journal	PLoS biology
Volume	22
Issue number	7 JULY
DOIs	https://doi.org/10.1371/journal.pbio.3002704
State	Published - Jul 2024

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Agricultural and Biological Sciences

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10.1371/journal.pbio.3002704

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Liu, Z., Liao, C., Zou, L., Jin, M., Shan, Y., Quan, Y., Yao, H., Zhang, L., Wang, P., Liu, Z., Wang, N., Li, A., Liu, K., Tabashnik, B. E., Heckel, D. G., Wu, K., & Xiao, Y. (2024). Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin. PLoS biology, 22(7 JULY), Article e3002704. https://doi.org/10.1371/journal.pbio.3002704

Liu, Z, Liao, C, Zou, L, Jin, M, Shan, Y, Quan, Y, Yao, H, Zhang, L, Wang, P, Liu, Z, Wang, N, Li, A, Liu, K, Tabashnik, BE, Heckel, DG, Wu, K & Xiao, Y 2024, 'Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin', PLoS biology, vol. 22, no. 7 JULY, e3002704. https://doi.org/10.1371/journal.pbio.3002704

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abstract = "The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has been produced by transgenic crops to counter pest resistance to the widely used crystalline (Cry) insecticidal proteins from Bt. To proactively manage pest resistance, there is an urgent need to better understand the genetic basis of resistance to Vip3Aa, which has been largely unknown. We discovered that retrotransposon-mediated alternative splicing of a midgut-specific chitin synthase gene was associated with 5,560-fold resistance to Vip3Aa in a laboratory-selected strain of the fall armyworm, a globally important crop pest. The same mutation in this gene was also detected in a field population. Knockout of this gene via CRISPR/ Cas9 caused high levels of resistance to Vip3Aa in fall armyworm and 2 other lepidopteran pests. The insights provided by these results could help to advance monitoring and management of pest resistance to Vip3Aa.",

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AU - Quan, Yudong

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N2 - The vegetative insecticidal protein Vip3Aa from Bacillus thuringiensis (Bt) has been produced by transgenic crops to counter pest resistance to the widely used crystalline (Cry) insecticidal proteins from Bt. To proactively manage pest resistance, there is an urgent need to better understand the genetic basis of resistance to Vip3Aa, which has been largely unknown. We discovered that retrotransposon-mediated alternative splicing of a midgut-specific chitin synthase gene was associated with 5,560-fold resistance to Vip3Aa in a laboratory-selected strain of the fall armyworm, a globally important crop pest. The same mutation in this gene was also detected in a field population. Knockout of this gene via CRISPR/ Cas9 caused high levels of resistance to Vip3Aa in fall armyworm and 2 other lepidopteran pests. The insights provided by these results could help to advance monitoring and management of pest resistance to Vip3Aa.

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Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin

Abstract

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