TY - JOUR
T1 - Retinoic acid biosynthetic activity and retinoid receptors in the olfactory mucosa of adult mice
AU - Zhang, Qing Yu
N1 - Funding Information:
This investigation was supported in part by Grant DC03355 from the National Institute on Deafness and Other Communication Disorders, National Institutes of Health. The author is grateful to Drs. Xinxin Ding and Laurence S. Kaminsky for reading the manuscript, Deborah Dunbar for assistance, and the Molecular Genetics Core of the Wadsworth Center for DNA sequence analysis.
PY - 1999/3/16
Y1 - 1999/3/16
N2 - Retinoids play important roles in the ontogenic development of the olfactory system in mammals, but their function in adult olfactory mucosa has not been explored. In the present study, the olfactory mucosal expression of nuclear retinoid receptors was examined in adult mice. Several retinoic acid receptor isotypes were identified in olfactory mucosa from adult C57BL/6 mice by RNA-PCR and DNA sequence analysis, including RARβ, RXRα, RXRβ, and RXRγ. In addition, a previously unidentified mouse RXRβ isoform containing a 12-nucleotide insertion in exon 7 was detected. Furthermore, in vitro metabolic studies demonstrated that olfactory mucosal cytosolic and microsomal preparations are active in the biosynthesis of retinoic acids from all-trans- and 9-cis-retinal. These results indicate that components of the retinoid signal transduction system are expressed in adult olfactory mucosa and may play important roles in gene regulation in this unique tissue where olfactory neuronal cells are continuously replaced.
AB - Retinoids play important roles in the ontogenic development of the olfactory system in mammals, but their function in adult olfactory mucosa has not been explored. In the present study, the olfactory mucosal expression of nuclear retinoid receptors was examined in adult mice. Several retinoic acid receptor isotypes were identified in olfactory mucosa from adult C57BL/6 mice by RNA-PCR and DNA sequence analysis, including RARβ, RXRα, RXRβ, and RXRγ. In addition, a previously unidentified mouse RXRβ isoform containing a 12-nucleotide insertion in exon 7 was detected. Furthermore, in vitro metabolic studies demonstrated that olfactory mucosal cytosolic and microsomal preparations are active in the biosynthesis of retinoic acids from all-trans- and 9-cis-retinal. These results indicate that components of the retinoid signal transduction system are expressed in adult olfactory mucosa and may play important roles in gene regulation in this unique tissue where olfactory neuronal cells are continuously replaced.
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U2 - 10.1006/bbrc.1999.0320
DO - 10.1006/bbrc.1999.0320
M3 - Article
C2 - 10079186
AN - SCOPUS:0033574269
SN - 0006-291X
VL - 256
SP - 346
EP - 351
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -