Response to NaCl of alfalfa plants regenerated from non-saline callus cultures

D. W. Johnson, S. E. Smith

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Salinity restricts crop productivity in many arid environments. Inadvertent selection for tolerance to osmotic stress may occur under cell or tissue culture conditions and could affect the performance of regenerated plants. The effect of NaCl on forage produced by alfalfa (Medicago sativa L.) plants regenerated from non-saline callus cultures was examined in this study. Plants of Regen-S, which was selected for improved callus growth and regeneration in non-saline cultures, had higher forage weight when grown on SHII medium at NaCl levels up to 100 m M compared to its parental cultivars, Saranac and DuPuits. Five additional original-regenerant plant pairs, each derived from non-saline callus cultures of different alfalfa plants, were evaluated in a solid (soil-like) substrate under saline and non-saline conditions. Weight of forage produced by rooted stem cuttings of regenerated plants was 33% higher at 50 m M NaCl compared to cuttings of explant donor plants. Self progenies from four of five regenerants had higher relative forage weight at 100 m M NaCl (percent of 0 NaCl treatment) than the original plants indicating increased NaCl tolerance.

Original languageEnglish (US)
Pages (from-to)311-315
Number of pages5
JournalPlant and Soil
Issue number2
StatePublished - Jun 1992


  • Medicago sativa
  • in vitro selection
  • lucerne
  • salinity

ASJC Scopus subject areas

  • Soil Science
  • Plant Science


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