TY - JOUR
T1 - Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness
AU - Kim, Dong Hwan
AU - Novak, Matt T.
AU - Wilkins, Jamie
AU - Kim, Minkyu
AU - Sawyer, Anita
AU - Reichert, William M.
N1 - Funding Information:
This research was supported by NIH (DK054932), the Whitaker Foundation, and Becton Dickinson and Company. The authors thank Rob Schutte for helpful discussions regarding cytokine profiling, and Jongmyeong Kim for the surface area calculations.
PY - 2007/10
Y1 - 2007/10
N2 - This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78±0.12 μm) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 μg/mL lipopolysaccharide (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1β, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-α, IL-6, MIP-1α, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles.
AB - This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78±0.12 μm) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 μg/mL lipopolysaccharide (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1β, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-α, IL-6, MIP-1α, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles.
KW - Cytokines
KW - Endotoxin
KW - Inflammation and wound healing
KW - Monocytes
KW - Ti
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U2 - 10.1016/j.biomaterials.2007.06.014
DO - 10.1016/j.biomaterials.2007.06.014
M3 - Article
C2 - 17631956
AN - SCOPUS:34547650982
SN - 0142-9612
VL - 28
SP - 4231
EP - 4239
JO - Biomaterials
JF - Biomaterials
IS - 29
ER -