Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets

Anouck L.S. Burzava; Aurelien Forget; Frances J. Harding; Michaelia P. Cockshell; Daniella Penko; Camille Rouzaud; Vincent Ahmadi; Paula F. Marina; Darling Rojas-Canales; Claudine S. Bonder; P. Toby H. Coates; Michaela Waibel; Helen E. Thomas; Thomas W. Kay; Thomas Loudovaris; Anton Blencowe; Nicolas H. Voelcker

doi:10.1116/1.5087737

Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets

Anouck L.S. Burzava, Aurelien Forget, Frances J. Harding, Michaelia P. Cockshell, Daniella Penko, Camille Rouzaud, Vincent Ahmadi, Paula F. Marina, Darling Rojas-Canales, Claudine S. Bonder, P. Toby H. Coates, Michaela Waibel, Helen E. Thomas, Thomas W. Kay, Thomas Loudovaris, Anton Blencowe, Nicolas H. Voelcker

Research output: Contribution to journal › Article › peer-review

Abstract

PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilometry that the topography of the 3D printed moulds can be transferred to the PMAs and that this roughness induced cell adhesive properties to the material. In particular, the topography facilitated immobilization of endothelial cells on the internal walls of the microwells. The authors also demonstrate that upon immobilization of endothelial cells to the microwells, a second population of cells, namely, pancreatic islets could be introduced, thus producing a 3D coculture platform.

Original language	English (US)
Article number	011002
Journal	Biointerphases
Volume	14
Issue number	1
DOIs	https://doi.org/10.1116/1.5087737
State	Published - Jan 1 2019
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
Biomaterials
General Materials Science
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1116/1.5087737

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Burzava, A. L. S., Forget, A., Harding, F. J., Cockshell, M. P., Penko, D., Rouzaud, C., Ahmadi, V., Marina, P. F., Rojas-Canales, D., Bonder, C. S., Coates, P. T. H., Waibel, M., Thomas, H. E., Kay, T. W., Loudovaris, T., Blencowe, A., & Voelcker, N. H. (2019). Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets. Biointerphases, 14(1), Article 011002. https://doi.org/10.1116/1.5087737

Burzava, ALS, Forget, A, Harding, FJ, Cockshell, MP, Penko, D, Rouzaud, C, Ahmadi, V, Marina, PF, Rojas-Canales, D, Bonder, CS, Coates, PTH, Waibel, M, Thomas, HE, Kay, TW, Loudovaris, T, Blencowe, A & Voelcker, NH 2019, 'Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets', Biointerphases, vol. 14, no. 1, 011002. https://doi.org/10.1116/1.5087737

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title = "Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets",

abstract = "PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilometry that the topography of the 3D printed moulds can be transferred to the PMAs and that this roughness induced cell adhesive properties to the material. In particular, the topography facilitated immobilization of endothelial cells on the internal walls of the microwells. The authors also demonstrate that upon immobilization of endothelial cells to the microwells, a second population of cells, namely, pancreatic islets could be introduced, thus producing a 3D coculture platform.",

author = "Burzava, {Anouck L.S.} and Aurelien Forget and Harding, {Frances J.} and Cockshell, {Michaelia P.} and Daniella Penko and Camille Rouzaud and Vincent Ahmadi and Marina, {Paula F.} and Darling Rojas-Canales and Bonder, {Claudine S.} and Coates, {P. Toby H.} and Michaela Waibel and Thomas, {Helen E.} and Kay, {Thomas W.} and Thomas Loudovaris and Anton Blencowe and Voelcker, {Nicolas H.}",

note = "Funding Information: This work was funded by the Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM). This work was performed in part at the South Australian and Victorian nodes of the Australian National Fabrication Facility under the National Collaborative Research Infrastructure Strategy. St Vincent{\textquoteright}s Institute receives support from the Operational Infrastructure Support Scheme of the Government of Victoria. The authors would like to thank Marta Krasowska for help with AFM analysis. Publisher Copyright: {\textcopyright} 2019 Author(s).",

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AU - Cockshell, Michaelia P.

AU - Penko, Daniella

AU - Rouzaud, Camille

AU - Ahmadi, Vincent

AU - Marina, Paula F.

AU - Rojas-Canales, Darling

AU - Bonder, Claudine S.

AU - Coates, P. Toby H.

AU - Waibel, Michaela

AU - Thomas, Helen E.

AU - Kay, Thomas W.

AU - Loudovaris, Thomas

AU - Blencowe, Anton

AU - Voelcker, Nicolas H.

N1 - Funding Information: This work was funded by the Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM). This work was performed in part at the South Australian and Victorian nodes of the Australian National Fabrication Facility under the National Collaborative Research Infrastructure Strategy. St Vincent’s Institute receives support from the Operational Infrastructure Support Scheme of the Government of Victoria. The authors would like to thank Marta Krasowska for help with AFM analysis. Publisher Copyright: © 2019 Author(s).

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Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets

Abstract

ASJC Scopus subject areas

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