TY - JOUR
T1 - Relaxation of Bovine Mesenteric Artery Induced by Glyceryl Trinitrate is Attenuated by Pertussis Toxin
AU - Ahlner, Johan
AU - Axelsson, Krister L.
AU - Ekstram‐Ljusegren, Marie
AU - Friedman, Richard L.
AU - Gnindström, Nils
AU - Karlsson, Ja Olof G.
AU - Andersson, Rolf G.G.
PY - 1988/3
Y1 - 1988/3
N2 - Abstract: Low concentrations (< 1 nM) of glyceryl trinitrate (GTN) induced a considerable relaxation of bovine mesenteric arteries (BMA) brought to sustained contraction by the addition of phenylephrine. The concentration‐response curve of GTN showed a biphasic pattern with a high and a low affinity component. Pretreatment with pertussis toxin (IAP) attenuated the high affinity relaxant component, but not the low affinity component or the relaxation induced by NaNO2. A polyclonal antibody to IAP counteracted the effect of the toxin on the GTN‐response. Low concentrations of GTN increased the cGMP level in BMA via activation of the high affinity pathway. This effect was also inhibited in preparations pretreated with IAP. It is suggested from the present study that GTN induces relaxation of vascular smooth muscle via two separate pathways, the high affinity pathway might involve a receptor‐complex interaction with a regulatory protein. 1988 Nordic Pharmacological Society
AB - Abstract: Low concentrations (< 1 nM) of glyceryl trinitrate (GTN) induced a considerable relaxation of bovine mesenteric arteries (BMA) brought to sustained contraction by the addition of phenylephrine. The concentration‐response curve of GTN showed a biphasic pattern with a high and a low affinity component. Pretreatment with pertussis toxin (IAP) attenuated the high affinity relaxant component, but not the low affinity component or the relaxation induced by NaNO2. A polyclonal antibody to IAP counteracted the effect of the toxin on the GTN‐response. Low concentrations of GTN increased the cGMP level in BMA via activation of the high affinity pathway. This effect was also inhibited in preparations pretreated with IAP. It is suggested from the present study that GTN induces relaxation of vascular smooth muscle via two separate pathways, the high affinity pathway might involve a receptor‐complex interaction with a regulatory protein. 1988 Nordic Pharmacological Society
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U2 - 10.1111/j.1600-0773.1988.tb01864.x
DO - 10.1111/j.1600-0773.1988.tb01864.x
M3 - Article
C2 - 2836843
AN - SCOPUS:0023832793
SN - 0901-9928
VL - 62
SP - 155
EP - 158
JO - Pharmacology & Toxicology
JF - Pharmacology & Toxicology
IS - 3
ER -