Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells

R. E. Whatley; K. L. Clay; F. H. Chilton; M. Triggiani; G. A. Zimmerman; T. M. McIntyre; S. M. Prescott

doi:10.1016/0090-6980(92)90061-W

Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells

R. E. Whatley, K. L. Clay, F. H. Chilton, M. Triggiani, G. A. Zimmerman, T. M. McIntyre, S. M. Prescott

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The specific precursor for platelet-activating factor, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, constitutes 10 per cent of the 1-radyl-2-acyl-sn-glycero-3-phosphocholines in endothelial cells. Stimulation of endothelial cells results in accumulation of PAF and its sn-1-acyl- analog (acylPAF), with acylPAF the predominant product. Mass spectrometry confirmed these relative amounts and confirmed that stimulated endothelial cells accumulate 1-3 ng PAF per million cells. These data suggest that stimulated endothelial cells accumulate both PAF and acylPAF and that the PAF synthetic pathway in endothelial cells is not highly selective for the specific PAF precursor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine).

Original language	English (US)
Pages (from-to)	21-29
Number of pages	9
Journal	Prostaglandins
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/0090-6980(92)90061-W
State	Published - Jan 1992
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology

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@article{285f3c510872492db24145c1eed16792,

title = "Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells",

abstract = "The specific precursor for platelet-activating factor, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, constitutes 10 per cent of the 1-radyl-2-acyl-sn-glycero-3-phosphocholines in endothelial cells. Stimulation of endothelial cells results in accumulation of PAF and its sn-1-acyl- analog (acylPAF), with acylPAF the predominant product. Mass spectrometry confirmed these relative amounts and confirmed that stimulated endothelial cells accumulate 1-3 ng PAF per million cells. These data suggest that stimulated endothelial cells accumulate both PAF and acylPAF and that the PAF synthetic pathway in endothelial cells is not highly selective for the specific PAF precursor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine).",

author = "Whatley, {R. E.} and Clay, {K. L.} and Chilton, {F. H.} and M. Triggiani and Zimmerman, {G. A.} and McIntyre, {T. M.} and Prescott, {S. M.}",

note = "Funding Information: This work was supported by the Veterans Affairs Medical Research Program, the Nora Eccles Treadwell Foundation. NIH Grants HL 43689. HL 34127. HL 35828. AI 24985, AI 26771 and a Grantiin-aid from the American Heart {\textquoteleft}Association (84-975). Guy Zimmerman and Stephen Prescott are recipients of Established Investigator Awards (Nos. 85-204 and 87-225) from the American Heart Association. Ralph Whatley has a Career Development Award from the Veterans Affairs Medical Research Program.",

year = "1992",

month = jan,

doi = "10.1016/0090-6980(92)90061-W",

language = "English (US)",

volume = "43",

pages = "21--29",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells

AU - Whatley, R. E.

AU - Clay, K. L.

AU - Chilton, F. H.

AU - Triggiani, M.

AU - Zimmerman, G. A.

AU - McIntyre, T. M.

AU - Prescott, S. M.

N1 - Funding Information: This work was supported by the Veterans Affairs Medical Research Program, the Nora Eccles Treadwell Foundation. NIH Grants HL 43689. HL 34127. HL 35828. AI 24985, AI 26771 and a Grantiin-aid from the American Heart ‘Association (84-975). Guy Zimmerman and Stephen Prescott are recipients of Established Investigator Awards (Nos. 85-204 and 87-225) from the American Heart Association. Ralph Whatley has a Career Development Award from the Veterans Affairs Medical Research Program.

PY - 1992/1

Y1 - 1992/1

N2 - The specific precursor for platelet-activating factor, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, constitutes 10 per cent of the 1-radyl-2-acyl-sn-glycero-3-phosphocholines in endothelial cells. Stimulation of endothelial cells results in accumulation of PAF and its sn-1-acyl- analog (acylPAF), with acylPAF the predominant product. Mass spectrometry confirmed these relative amounts and confirmed that stimulated endothelial cells accumulate 1-3 ng PAF per million cells. These data suggest that stimulated endothelial cells accumulate both PAF and acylPAF and that the PAF synthetic pathway in endothelial cells is not highly selective for the specific PAF precursor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine).

AB - The specific precursor for platelet-activating factor, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, constitutes 10 per cent of the 1-radyl-2-acyl-sn-glycero-3-phosphocholines in endothelial cells. Stimulation of endothelial cells results in accumulation of PAF and its sn-1-acyl- analog (acylPAF), with acylPAF the predominant product. Mass spectrometry confirmed these relative amounts and confirmed that stimulated endothelial cells accumulate 1-3 ng PAF per million cells. These data suggest that stimulated endothelial cells accumulate both PAF and acylPAF and that the PAF synthetic pathway in endothelial cells is not highly selective for the specific PAF precursor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine).

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Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells

Abstract

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