Relative abundance of bovine Hsp70 mRNA and protein

Jesus A. Gutierrez; Vince Guerriero

doi:10.1016/0167-4781(94)00220-W

Relative abundance of bovine Hsp70 mRNA and protein

Jesus A. Gutierrez, Vince Guerriero

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A solution hybridization assay to measure bovine Hsp70 mRNA levels is used to demonstrate that skeletal muscle contains the highest amount of this mRNA while brain has the lowest amount and the level of Hsp70 mRNA is heat inducible in bovine skeletal muscle. Furthermore, this assay allowed for the comparison of relative Hsp70 protein and mRNA levels. Relative transcript levels compared to relative Hsp70 protein levels in tissues demonstrated 50- to 200-fold differences. These results, then, approximate the magnitude of the previously reported preferential translation of Hsp70 mRNA.

Original language	English (US)
Pages (from-to)	239-242
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1260
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(94)00220-W
State	Published - Jan 25 1995

Keywords

(Bovine)
Hsp70
Protein level
mRNA level

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(94)00220-W

Cite this

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title = "Relative abundance of bovine Hsp70 mRNA and protein",

abstract = "A solution hybridization assay to measure bovine Hsp70 mRNA levels is used to demonstrate that skeletal muscle contains the highest amount of this mRNA while brain has the lowest amount and the level of Hsp70 mRNA is heat inducible in bovine skeletal muscle. Furthermore, this assay allowed for the comparison of relative Hsp70 protein and mRNA levels. Relative transcript levels compared to relative Hsp70 protein levels in tissues demonstrated 50- to 200-fold differences. These results, then, approximate the magnitude of the previously reported preferential translation of Hsp70 mRNA.",

keywords = "(Bovine), Hsp70, Protein level, mRNA level",

author = "Gutierrez, {Jesus A.} and Vince Guerriero",

note = "Funding Information: This research was supported by grants from the American Heart Association, Arizona Affiliate (AZ G-1-93) and from NIH (GM14852).",

year = "1995",

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doi = "10.1016/0167-4781(94)00220-W",

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pages = "239--242",

journal = "BBA - Gene Structure and Expression",

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TY - JOUR

T1 - Relative abundance of bovine Hsp70 mRNA and protein

AU - Gutierrez, Jesus A.

AU - Guerriero, Vince

N1 - Funding Information: This research was supported by grants from the American Heart Association, Arizona Affiliate (AZ G-1-93) and from NIH (GM14852).

PY - 1995/1/25

Y1 - 1995/1/25

N2 - A solution hybridization assay to measure bovine Hsp70 mRNA levels is used to demonstrate that skeletal muscle contains the highest amount of this mRNA while brain has the lowest amount and the level of Hsp70 mRNA is heat inducible in bovine skeletal muscle. Furthermore, this assay allowed for the comparison of relative Hsp70 protein and mRNA levels. Relative transcript levels compared to relative Hsp70 protein levels in tissues demonstrated 50- to 200-fold differences. These results, then, approximate the magnitude of the previously reported preferential translation of Hsp70 mRNA.

AB - A solution hybridization assay to measure bovine Hsp70 mRNA levels is used to demonstrate that skeletal muscle contains the highest amount of this mRNA while brain has the lowest amount and the level of Hsp70 mRNA is heat inducible in bovine skeletal muscle. Furthermore, this assay allowed for the comparison of relative Hsp70 protein and mRNA levels. Relative transcript levels compared to relative Hsp70 protein levels in tissues demonstrated 50- to 200-fold differences. These results, then, approximate the magnitude of the previously reported preferential translation of Hsp70 mRNA.

KW - (Bovine)

KW - Hsp70

KW - Protein level

KW - mRNA level

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U2 - 10.1016/0167-4781(94)00220-W

DO - 10.1016/0167-4781(94)00220-W

M3 - Article

C2 - 7841204

AN - SCOPUS:0028897309

SN - 0167-4781

VL - 1260

SP - 239

EP - 242

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 2

ER -

Relative abundance of bovine Hsp70 mRNA and protein

Abstract

Keywords

ASJC Scopus subject areas

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