TY - JOUR
T1 - Relation of potassium to urate accumulation by kidney slices from desert spiny lizards (Sceloporus magister)
AU - Dantzler, William H.
N1 - Funding Information:
Acknowledgements-I thank Mr. James F. Kiernat and Mrs. Maquerite Witte for their technical assistance. This investigation was supported by Nationai Science Foundation Research Grant No. GB-11788.
PY - 1971/10/1
Y1 - 1971/10/1
N2 - 1. 1. The relation of medium potassium concentration to urate accumulation by kidney slices from desert spiny lizards (Sceloporus magister) was studied. 2. 2. Incubation in 40 mM potassium caused a marked increase in urate accumulation. Preparation and incubation in potassium-free medium depressed, but did not eliminate, active urate accumulation. 3. 3. Incubation of potassium-depleted slices in media of increasing potassium concentration restored tissue potassium when medium potassium reached 40 mM but did not completely restore urate accumulation. 4. 4. These data suggest that severe depletion of potassium impairs mechanisms for both potassium and urate accumulation, and that the latter is not restored even when tissue potassium is restored. 5. 5. These results differ from those observed previously with kidney slices from domestic fowl and ophidian reptiles. Differences may be related to the membrane site of transport.
AB - 1. 1. The relation of medium potassium concentration to urate accumulation by kidney slices from desert spiny lizards (Sceloporus magister) was studied. 2. 2. Incubation in 40 mM potassium caused a marked increase in urate accumulation. Preparation and incubation in potassium-free medium depressed, but did not eliminate, active urate accumulation. 3. 3. Incubation of potassium-depleted slices in media of increasing potassium concentration restored tissue potassium when medium potassium reached 40 mM but did not completely restore urate accumulation. 4. 4. These data suggest that severe depletion of potassium impairs mechanisms for both potassium and urate accumulation, and that the latter is not restored even when tissue potassium is restored. 5. 5. These results differ from those observed previously with kidney slices from domestic fowl and ophidian reptiles. Differences may be related to the membrane site of transport.
KW - Organic acid transport
KW - desert spiny lizard Sceloporus magister
KW - lizard kidney slices
KW - potassium and urate transport
KW - renal tubular transport
KW - urate transport
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U2 - 10.1016/0300-9629(71)90038-7
DO - 10.1016/0300-9629(71)90038-7
M3 - Article
C2 - 4400944
AN - SCOPUS:0015139329
SN - 0300-9629
VL - 40
SP - 467
EP - 477
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
IS - 2
ER -