TY - JOUR
T1 - Regulation of poly(A) site choice of several yeast mRNAs
AU - Sparks, Kimberly A.
AU - Dieckmann, Carol L.
N1 - Funding Information:
The authors wish to thank the following people: Elisabeth Mandart and François Lacroute for the gift of plasmid pLM4; Elisa Stone and Lorraine Pillus for the gift of plasmid pLP75; Sharon Ackerman for the gift of plasmid pG95/ST3-2; Barbara Hoopes, Annette M. Krecic and Lionel Minvielle-Sebastia for sharing unpublished results; Steve Mayer for preparing the poly(A)+ RNA used in the northern blot analysis; Cathy Kingdon Worley for help with Figure 5; Doug Roberts and Laurie Marnell for critical reading of the manuscript. This study was supported by National Institutes of Health grant GM34893 to C.L.D.
PY - 1998/10/15
Y1 - 1998/10/15
N2 - Several yeast genes produce multiple transcripts with different 3'-ends. Of these, four genes are known to produce truncated transcripts that end within the coding sequence of longer transcripts: CBP1, AEP2/ATP13, RNA 14 and SIR1. It has been shown that the level of the truncated CBP1 transcript increases during the switch to respiratory growth while that of the full-length transcript decreases. To determine whether this phenomenon is unique to CBP1, northern analysis was used to determine whether the levels of other truncated transcripts are regulated similarly by carbon source. The levels of the shortest transcripts of AEP2/ATP13 and RNA 14 increased during respiration while the shortest SIR1 transcript remained constant. However, two longer SIR1 transcripts were regulated reciprocally by carbon source. Mapping the 3'-ends of each transcript by sequencing partial cDNA clones revealed multiple 3'-ends for each transcript. Examination of the sequences surrounding the 3'-ends of the induced transcripts failed to identify a consensus sequence but did reveal weak putative 3'end formation signals in all of the transcripts. Similarly, no consensus sequence was found when the sequences surrounding the 3'-ends of the longest transcripts were compared, but again weak putative 3'-end formation signals were identified. These data are suggestive of carbon source regulation of alternative poly(A) site choice in yeast.
AB - Several yeast genes produce multiple transcripts with different 3'-ends. Of these, four genes are known to produce truncated transcripts that end within the coding sequence of longer transcripts: CBP1, AEP2/ATP13, RNA 14 and SIR1. It has been shown that the level of the truncated CBP1 transcript increases during the switch to respiratory growth while that of the full-length transcript decreases. To determine whether this phenomenon is unique to CBP1, northern analysis was used to determine whether the levels of other truncated transcripts are regulated similarly by carbon source. The levels of the shortest transcripts of AEP2/ATP13 and RNA 14 increased during respiration while the shortest SIR1 transcript remained constant. However, two longer SIR1 transcripts were regulated reciprocally by carbon source. Mapping the 3'-ends of each transcript by sequencing partial cDNA clones revealed multiple 3'-ends for each transcript. Examination of the sequences surrounding the 3'-ends of the induced transcripts failed to identify a consensus sequence but did reveal weak putative 3'end formation signals in all of the transcripts. Similarly, no consensus sequence was found when the sequences surrounding the 3'-ends of the longest transcripts were compared, but again weak putative 3'-end formation signals were identified. These data are suggestive of carbon source regulation of alternative poly(A) site choice in yeast.
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U2 - 10.1093/nar/26.20.4676
DO - 10.1093/nar/26.20.4676
M3 - Article
C2 - 9753737
AN - SCOPUS:0032531703
VL - 26
SP - 4676
EP - 4687
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 20
ER -