Regulation of human papillomavirus 18 genome replication, establishment, and persistence by sequences in the viral upstream regulatory region

Tami L. Coursey, Koenraad Van Doorslaer, Alison A. McBride

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

During persistent human papillomavirus infection, the viral genome replicates as an extrachromosomal plasmid that is efficiently partitioned to daughter cells during cell division. We have previously shown that an element which overlaps the human papillomavirus 18 (HPV18) transcriptional enhancer promotes stable DNA replication of replicons containing the viral replication origin. Here, we perform comprehensive analyses to elucidate the function of this maintenance element. We conclude that no unique element or binding site in this region is absolutely required for persistent replication and partitioning and instead propose that the overall chromatin architecture of this region is important to promote efficient use of the replication origin. These results have important implications for the genome partitioning mechanism of papillomaviruses.

Original languageEnglish (US)
Article numbere00686-21
JournalJournal of virology
Volume95
Issue number19
DOIs
StatePublished - Oct 2021

Keywords

  • AP1
  • Alphapapillomavirus
  • C-MYC
  • Enhancer
  • HPV
  • HPV18
  • Maintenance
  • Papillomavirus
  • Partitioning
  • Persistence
  • Persistent infection
  • Replication

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint

Dive into the research topics of 'Regulation of human papillomavirus 18 genome replication, establishment, and persistence by sequences in the viral upstream regulatory region'. Together they form a unique fingerprint.

Cite this