Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol

W. L. Miller; D. C. Alexander; J. C. Wu; E. S. Huang; G. K. Whitfield; S. H. Hall

doi:10.1007/BF00225253

Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol

W. L. Miller, D. C. Alexander, J. C. Wu, E. S. Huang, G. K. Whitfield, S. H. Hall

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

17β-Estradiol (E₂) rapidly and reversibly decreases the synthesis of follicle-stimulating hormone (FSH) in primary dispersed cell cultures of ovine pituitaries. Similarly, E2 also causes a decrease in the messenger RNA for the beta subunit of ovine FSH (FSIIβ-mRNA) as measured in an in vitro translation assay. These results are consistent with the concept that E₂ directly regulates either transcription of the FSHβ gene or processing of FSHβ-mRNA in sheep. Of additional interest is the observation that pituitary cultures from various species respond differently to E₂ in terms of FSH synthesis. For example, E₂ stimulates FSH synthesis in rat pituitary cultures, has no effect in similar rabbit cultures, and inhibits FSH synthesis in ovine cultures. Thus, a set of eukaryotic 'mutants' may exist to aid studies of the effect of E₂ on FSH synthesis and secretion.

Original language	English (US)
Pages (from-to)	187-195
Number of pages	9
Journal	Molecular and Cellular Biochemistry
Volume	53-54
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00225253
State	Published - Mar 1983
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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title = "Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol",

abstract = "17β-Estradiol (E2) rapidly and reversibly decreases the synthesis of follicle-stimulating hormone (FSH) in primary dispersed cell cultures of ovine pituitaries. Similarly, E2 also causes a decrease in the messenger RNA for the beta subunit of ovine FSH (FSIIβ-mRNA) as measured in an in vitro translation assay. These results are consistent with the concept that E2 directly regulates either transcription of the FSHβ gene or processing of FSHβ-mRNA in sheep. Of additional interest is the observation that pituitary cultures from various species respond differently to E2 in terms of FSH synthesis. For example, E2 stimulates FSH synthesis in rat pituitary cultures, has no effect in similar rabbit cultures, and inhibits FSH synthesis in ovine cultures. Thus, a set of eukaryotic 'mutants' may exist to aid studies of the effect of E2 on FSH synthesis and secretion.",

author = "Miller, {W. L.} and Alexander, {D. C.} and Wu, {J. C.} and Huang, {E. S.} and Whitfield, {G. K.} and Hall, {S. H.}",

year = "1983",

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T1 - Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol

AU - Miller, W. L.

AU - Alexander, D. C.

AU - Wu, J. C.

AU - Huang, E. S.

AU - Whitfield, G. K.

AU - Hall, S. H.

PY - 1983/3

Y1 - 1983/3

N2 - 17β-Estradiol (E2) rapidly and reversibly decreases the synthesis of follicle-stimulating hormone (FSH) in primary dispersed cell cultures of ovine pituitaries. Similarly, E2 also causes a decrease in the messenger RNA for the beta subunit of ovine FSH (FSIIβ-mRNA) as measured in an in vitro translation assay. These results are consistent with the concept that E2 directly regulates either transcription of the FSHβ gene or processing of FSHβ-mRNA in sheep. Of additional interest is the observation that pituitary cultures from various species respond differently to E2 in terms of FSH synthesis. For example, E2 stimulates FSH synthesis in rat pituitary cultures, has no effect in similar rabbit cultures, and inhibits FSH synthesis in ovine cultures. Thus, a set of eukaryotic 'mutants' may exist to aid studies of the effect of E2 on FSH synthesis and secretion.

AB - 17β-Estradiol (E2) rapidly and reversibly decreases the synthesis of follicle-stimulating hormone (FSH) in primary dispersed cell cultures of ovine pituitaries. Similarly, E2 also causes a decrease in the messenger RNA for the beta subunit of ovine FSH (FSIIβ-mRNA) as measured in an in vitro translation assay. These results are consistent with the concept that E2 directly regulates either transcription of the FSHβ gene or processing of FSHβ-mRNA in sheep. Of additional interest is the observation that pituitary cultures from various species respond differently to E2 in terms of FSH synthesis. For example, E2 stimulates FSH synthesis in rat pituitary cultures, has no effect in similar rabbit cultures, and inhibits FSH synthesis in ovine cultures. Thus, a set of eukaryotic 'mutants' may exist to aid studies of the effect of E2 on FSH synthesis and secretion.

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Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol

Abstract

ASJC Scopus subject areas

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