Regions of microsynteny in Magnaporthe grisea and Neurospora crassa

Lisbeth Hamer; Huaqin Pan; Kiichi Adachi; Marc J. Orbach; Amy Page; Lakshman Ramamurthy; Jeffrey P. Woessner

doi:10.1006/fgbi.2001.1286

Regions of microsynteny in Magnaporthe grisea and Neurospora crassa

Lisbeth Hamer
, Huaqin Pan
, Kiichi Adachi
, Marc J. Orbach
, Amy Page
, Lakshman Ramamurthy
, Jeffrey P. Woessner

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for ∼200 million years elicits many biological questions as to the function and identity of these genes.

Original language	English (US)
Pages (from-to)	137-143
Number of pages	7
Journal	Fungal Genetics and Biology
Volume	33
Issue number	2
DOIs	https://doi.org/10.1006/fgbi.2001.1286
State	Published - 2001

Keywords

Magnaporthe grisea
Microsynteny
Neurospora crassa
Rice blast fungus

ASJC Scopus subject areas

Microbiology
Genetics

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10.1006/fgbi.2001.1286

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title = "Regions of microsynteny in Magnaporthe grisea and Neurospora crassa",

abstract = "A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for ∼200 million years elicits many biological questions as to the function and identity of these genes.",

keywords = "Magnaporthe grisea, Microsynteny, Neurospora crassa, Rice blast fungus",

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year = "2001",

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AU - Hamer, Lisbeth

AU - Pan, Huaqin

AU - Adachi, Kiichi

AU - Orbach, Marc J.

AU - Page, Amy

AU - Ramamurthy, Lakshman

AU - Woessner, Jeffrey P.

PY - 2001

Y1 - 2001

N2 - A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for ∼200 million years elicits many biological questions as to the function and identity of these genes.

AB - A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for ∼200 million years elicits many biological questions as to the function and identity of these genes.

KW - Magnaporthe grisea

KW - Microsynteny

KW - Neurospora crassa

KW - Rice blast fungus

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Regions of microsynteny in Magnaporthe grisea and Neurospora crassa

Abstract

Keywords

ASJC Scopus subject areas

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