TY - JOUR
T1 - Regional distribution of putative vasopressin receptors in rat brain and pituitary by quantitative autoradiography
AU - Brinton, R. E.
AU - Gee, K. W.
AU - Wamsley, J. K.
AU - Davis, T. P.
AU - Yamamura, H. I.
PY - 1984
Y1 - 1984
N2 - Quantitative light microscopic autoradiography was used to map and characterize the distribution of [3H]arginine vasopressin ([3H]AVP) binding sites in the rat brain. HPLC analysis for possible degradation of AVP during binding indicated that addition of specific peptidase inhibitors prevented metabolism of AVP. Binding sites for [3H]AVP were observed in the hypothalamus and pituitary as well as in brain regions where AVP may act as a neuroregulator. Within the hypothalamus, dense AVP binding sites were seen in the suprachiasmatic, supraoptic, and paraventricular nuclei. High specific binding was also apparent in the median eminence tubero-infundibular region and in the posterior lobe of the pituitary. [3H]AVP labeling at possible neuroregulatory sites was observed in the hippocampus, lateral septum, superficial cortex, cerebellum, nucleus tractus solitarious, adenohypophysis, and spinal cord.
AB - Quantitative light microscopic autoradiography was used to map and characterize the distribution of [3H]arginine vasopressin ([3H]AVP) binding sites in the rat brain. HPLC analysis for possible degradation of AVP during binding indicated that addition of specific peptidase inhibitors prevented metabolism of AVP. Binding sites for [3H]AVP were observed in the hypothalamus and pituitary as well as in brain regions where AVP may act as a neuroregulator. Within the hypothalamus, dense AVP binding sites were seen in the suprachiasmatic, supraoptic, and paraventricular nuclei. High specific binding was also apparent in the median eminence tubero-infundibular region and in the posterior lobe of the pituitary. [3H]AVP labeling at possible neuroregulatory sites was observed in the hippocampus, lateral septum, superficial cortex, cerebellum, nucleus tractus solitarious, adenohypophysis, and spinal cord.
UR - http://www.scopus.com/inward/record.url?scp=0001081952&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001081952&partnerID=8YFLogxK
U2 - 10.1073/pnas.81.22.7248
DO - 10.1073/pnas.81.22.7248
M3 - Article
C2 - 6095279
AN - SCOPUS:0001081952
SN - 0027-8424
VL - 81
SP - 7248
EP - 7252
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22 I
ER -