Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney

Frank C. Brosius; Khanh Nguyen; A. K. Stuart-Tilley; C. Haller; Josephine P. Briggs; S. L. Alper

Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney

Frank C. Brosius, Khanh Nguyen, A. K. Stuart-Tilley, C. Haller, Josephine P. Briggs, S. L. Alper

Research output: Contribution to journal › Article › peer-review

Abstract

Chloride/ base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl^-/HCO₃^- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.

Original language	English (US)
Pages (from-to)	F461-F468
Journal	American Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume	269
Issue number	4 38-4
State	Published - Oct 1995
Externally published	Yes

Keywords

Antibody
Band 3
Cell lines
Chloride/bicarbonate exchange
Immunoblot
Microdissection
Northern blot
Polymerase chain reaction
Recombinant deoxyribonucleic acid
Reverse transcriptase

ASJC Scopus subject areas

Physiology

Cite this

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title = "Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney",

abstract = "Chloride/ base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl-/HCO3- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.",

keywords = "Antibody, Band 3, Cell lines, Chloride/bicarbonate exchange, Immunoblot, Microdissection, Northern blot, Polymerase chain reaction, Recombinant deoxyribonucleic acid, Reverse transcriptase",

author = "Brosius, {Frank C.} and Khanh Nguyen and Stuart-Tilley, {A. K.} and C. Haller and Briggs, {Josephine P.} and Alper, {S. L.}",

year = "1995",

month = oct,

language = "English (US)",

volume = "269",

pages = "F461--F468",

journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",

issn = "0363-6127",

publisher = "American Physiological Society",

number = "4 38-4",

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TY - JOUR

T1 - Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney

AU - Brosius, Frank C.

AU - Nguyen, Khanh

AU - Stuart-Tilley, A. K.

AU - Haller, C.

AU - Briggs, Josephine P.

AU - Alper, S. L.

PY - 1995/10

Y1 - 1995/10

N2 - Chloride/ base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl-/HCO3- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.

AB - Chloride/ base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl-/HCO3- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.

KW - Antibody

KW - Band 3

KW - Cell lines

KW - Chloride/bicarbonate exchange

KW - Immunoblot

KW - Microdissection

KW - Northern blot

KW - Polymerase chain reaction

KW - Recombinant deoxyribonucleic acid

KW - Reverse transcriptase

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M3 - Article

C2 - 7485530

AN - SCOPUS:0028842462

SN - 0363-6127

VL - 269

SP - F461-F468

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

IS - 4 38-4

ER -

Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney

Abstract

Keywords

ASJC Scopus subject areas

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