TY - JOUR
T1 - Regional and segmental localization of AE2 anion exchanger mRNA and protein in rat kidney
AU - Brosius, F. C.
AU - Nguyen, K.
AU - Stuart-Tilley, A. K.
AU - Haller, C.
AU - Briggs, J. P.
AU - Alper, S. L.
PY - 1995
Y1 - 1995
N2 - Chloride/base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl- /HCO3/- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.
AB - Chloride/base exchange activity has been detected in every mammalian nephron segment in which it has been sought. However, in contrast to the Cl- /HCO3/- exchanger AE1 in type A intercalated cells, localization of AE2 within the kidney has not been reported. We therefore studied AE2 expression in rat kidney. AE2 mRNA was present in cortex, outer medulla, and inner medulla. Semiquantitative polymerase chain reaction of cDNA from microdissected tubules revealed AE2 cDNA levels as follows [copies of cDNA derived per mm tubule (±SE)]: proximal convoluted tubule, 688 ± 161; proximal straight tubule, 652 ± 189; medullary thick ascending limb, 1,378 ± 226; cortical thick ascending limb, 741 ± 24; cortical collecting duct, 909 ± 71; and outer medullary collecting duct, 579 ± 132. AE2 cDNA was also amplified in thin limbs and in inner medullary collecting duct. AE2 polypeptide was detected in all kidney regions. AE2 mRNA and protein were also detected in several renal cell lines. The data are compatible with the postulated roles of AE2 in maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.
KW - Northern blot
KW - antibody
KW - band 3
KW - cell lines
KW - chloride/bicarbonate exchange
KW - immunoblot
KW - microdissection
KW - polymerase chain reaction
KW - recombinant deoxyribonucleic acid
KW - reverse transcriptase
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U2 - 10.1152/ajprenal.1995.269.4.f461
DO - 10.1152/ajprenal.1995.269.4.f461
M3 - Article
C2 - 7485530
AN - SCOPUS:0028842462
VL - 269
SP - F461-F468
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 4 38-4
ER -