Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

Michael D. Burkart; Masayuki Izumi; Eli Chapman; Chun Hung Lin; Chi Huey Wong

doi:10.1021/jo000266o

Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

Michael D. Burkart, Masayuki Izumi, Eli Chapman, Chun Hung Lin, Chi Huey Wong

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyl-transferases.

Original language	English (US)
Pages (from-to)	5565-5574
Number of pages	10
Journal	Journal of Organic Chemistry
Volume	65
Issue number	18
DOIs	https://doi.org/10.1021/jo000266o
State	Published - Sep 8 2000
Externally published	Yes

ASJC Scopus subject areas

Organic Chemistry

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10.1021/jo000266o

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abstract = "This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyl-transferases.",

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AU - Burkart, Michael D.

AU - Izumi, Masayuki

AU - Chapman, Eli

AU - Lin, Chun Hung

AU - Wong, Chi Huey

PY - 2000/9/8

Y1 - 2000/9/8

N2 - This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyl-transferases.

AB - This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyl-transferases.

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Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

Abstract

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