TY - GEN
T1 - Reflectance full Mueller matrix polarimetry for microstructural validation of diffusion magnetic resonance imaging
AU - Bonaventura, Justina
AU - Morara, Kellys
AU - Carlson, Rhea
AU - Comrie, Courtney
AU - Hutchinson, Elizabeth
AU - Sawyer, Travis W.
N1 - Publisher Copyright:
© 2023 SPIE.
PY - 2023
Y1 - 2023
N2 - Knowledge of fiber microstructure and orientation in the brain is critical for understanding the pathogenesis and progression of neurodegenerative diseases such as Alzheimer's Disease. Diffusion magnetic resonance imaging (dMRI) is a noninvasive imaging modality that can generate mappings of nerve fiber orientation. Due to rigorous levels of mathematical modeling involved in reconstructing dMRI data; and limited spatial resolution, there arises a need to validate the biological accuracy of collected dMRI data. Polarized light imaging (PLI) has been shown to have potential for microstructural validation due to the anisotropy in many biological tissues, particularly in myelin sheaths surrounding nerve fibers in the brain. Using PLI for this purpose is appealing because it is directly sensitive to tissue structure and can be done at high resolution. While several studies have had success using PLI for fiber mapping, continuing to advance this modality, particularly reflectance based PLI systems, could provide a valuable avenue for in vivo neural imaging. In order to reach the full potential of reflectance PLI systems, some key questions remain such as the ability of PLI to resolve crossing fibers, and the sensitivity of reflectance PLI to fiber inclination. Tissue phantoms are one potential method to isolate these issues in order to investigate them. In this proceeding, a five-wavelength reflectance Mueller Matrix polarimeter is used for imaging of promising PLI tissue phantoms as well as regions of interest in fixed ferret brain samples. The retardance, diattenuation and depolarization mappings are derived from the Mueller matrix and studied in order to assess the sensitivity of this polarimeter configuration to different fiber orientations.
AB - Knowledge of fiber microstructure and orientation in the brain is critical for understanding the pathogenesis and progression of neurodegenerative diseases such as Alzheimer's Disease. Diffusion magnetic resonance imaging (dMRI) is a noninvasive imaging modality that can generate mappings of nerve fiber orientation. Due to rigorous levels of mathematical modeling involved in reconstructing dMRI data; and limited spatial resolution, there arises a need to validate the biological accuracy of collected dMRI data. Polarized light imaging (PLI) has been shown to have potential for microstructural validation due to the anisotropy in many biological tissues, particularly in myelin sheaths surrounding nerve fibers in the brain. Using PLI for this purpose is appealing because it is directly sensitive to tissue structure and can be done at high resolution. While several studies have had success using PLI for fiber mapping, continuing to advance this modality, particularly reflectance based PLI systems, could provide a valuable avenue for in vivo neural imaging. In order to reach the full potential of reflectance PLI systems, some key questions remain such as the ability of PLI to resolve crossing fibers, and the sensitivity of reflectance PLI to fiber inclination. Tissue phantoms are one potential method to isolate these issues in order to investigate them. In this proceeding, a five-wavelength reflectance Mueller Matrix polarimeter is used for imaging of promising PLI tissue phantoms as well as regions of interest in fixed ferret brain samples. The retardance, diattenuation and depolarization mappings are derived from the Mueller matrix and studied in order to assess the sensitivity of this polarimeter configuration to different fiber orientations.
KW - Diffusion Magnetic Resonance Imaging
KW - Mueller Matrix Polarimetry
KW - Polarized Light Imaging
KW - Reflectance Polarimetry
KW - Tissue Phantoms
UR - http://www.scopus.com/inward/record.url?scp=85159786738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85159786738&partnerID=8YFLogxK
U2 - 10.1117/12.2650895
DO - 10.1117/12.2650895
M3 - Conference contribution
AN - SCOPUS:85159786738
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
A2 - Ramella-Roman, Jessica C.
A2 - Ma, Hui
A2 - Novikova, Tatiana
A2 - Elson, Daniel S.
A2 - Vitkin, I. Alex
PB - SPIE
T2 - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023
Y2 - 28 January 2023 through 29 January 2023
ER -