Reductive degradation behavior of bioreducible poly(disulfide amine) for enhancing SiRNA efficiency

Sun Hwa Kim, Mei Ou, David A. Bull, Sung Wan Kim

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Bioreducible cationic polymer poly(CBA-DAH) containing repeated disulfide linkages on the polymer backbone was synthesized through Michael-type polyadditions of CBA to DAH monomers. Poly(CBA-DAH) could spontaneously form nanoscale polyelectrolyte complexes through electrostatic interactions with siRNA in an aqueous phase. These nanoparticles were rapidly degraded under the reductive cytoplasmic environment with subsequently releasing the siRNA cargo into the cytoplasm where RNAi takes place, as a result of the breakdown of disulfide bonds in the polymers. The reductive degradation behavior of the poly(CBA-DAH)/ siRNA polyplexes is more likely to increase RNAi activity with enhancing the cytoplasmic localization of siRNA molecules. Poly(CBA-DAH) may have great potential as a gene carrier especially for therapeutic applications of siRNAs owing to the reductive degradation characteristics. (Figure Presented)

Original languageEnglish (US)
Pages (from-to)898-905
Number of pages8
JournalMacromolecular Bioscience
Volume10
Issue number8
DOIs
StatePublished - Aug 11 2010
Externally publishedYes

Keywords

  • Biodegradability
  • Bioreducible cationic polymer
  • Cytoplasmic localization
  • Nanoparticles
  • Polyelectrolyte complexes
  • Polyelectrolytes
  • siRNA delivery

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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