Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

Nancy A. Buchmeier; Craig J. Lipps; Magdalene Y.H. So; Fred Heffron

doi:10.1111/j.1365-2958.1993.tb01184.x

Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

Nancy A. Buchmeier, Craig J. Lipps, Magdalene Y.H. So, Fred Heffron

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147 Scopus citations

Abstract

Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.

Original language	English (US)
Pages (from-to)	933-936
Number of pages	4
Journal	Molecular Microbiology
Volume	7
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-2958.1993.tb01184.x
State	Published - Mar 1993
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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abstract = "Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.",

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AU - Buchmeier, Nancy A.

AU - Lipps, Craig J.

AU - So, Magdalene Y.H.

AU - Heffron, Fred

PY - 1993/3

Y1 - 1993/3

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AB - Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.

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Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

Abstract

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