TY - JOUR
T1 - Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells
AU - Haussler, M. R.
AU - Manolagas, S. C.
AU - Deftos, L. J.
N1 - Funding Information:
Acknowledgements-This work was supportedb y grants from the National Institutes of Health, the American CancerS ociety,t he VeteransA dministrationa, nd the Alex-anderO nassisF oundation.M . R. Hausslerw as a Faculty Scholar of the Josiah Macy, Jr. Foundation (1979-1980).
PY - 1982/1
Y1 - 1982/1
N2 - Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.
AB - Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.
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U2 - 10.1016/0022-4731(82)90138-8
DO - 10.1016/0022-4731(82)90138-8
M3 - Article
C2 - 6278224
AN - SCOPUS:0020048029
SN - 0960-0760
VL - 16
SP - 15
EP - 19
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 1
ER -