TY - JOUR
T1 - Recent developments in microarray-based enzyme assays
T2 - From functional annotation to substrate/inhibitor fingerprinting
AU - Sun, Hongyan
AU - Chattopadhaya, Souvik
AU - Wang, Jun
AU - Yao, Shao Q.
N1 - Funding Information:
Acknowledgements Funding support was provided by the National University of Singapore (NUS) and the Agency for Science, Technology and Research (A*STAR) of Singapore.
Funding Information:
is currently Associate Professor at the Departments of Chemistry and Biological Sciences at the National University of Singapore. He is the recipient of the 2002 Young Investi gator Award funded by the Biomed ical Research Council (BMRC) of the Singaporean government, and the 2005 Young Researcher Award (NUS). His research interests include combinatorial chemistry, chemical biology, proteomics and bioimaging.
PY - 2006/10
Y1 - 2006/10
N2 - Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term "catalomics" defines an emerging '-omics' field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.
AB - Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term "catalomics" defines an emerging '-omics' field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.
KW - Activity-based fingerprinting
KW - Activity-based probe
KW - Catalomics
KW - Drug discovery
KW - Enzyme assay
KW - Inhibitor fingerprinting
KW - Microarray
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U2 - 10.1007/s00216-006-0511-5
DO - 10.1007/s00216-006-0511-5
M3 - Article
C2 - 16791553
AN - SCOPUS:33749037968
SN - 1618-2642
VL - 386
SP - 416
EP - 426
JO - Analytical and bioanalytical chemistry
JF - Analytical and bioanalytical chemistry
IS - 3
ER -