Reagentless detection of microorganisms by intrinsic fluorescence

Cory Estes; Andrew Duncan; Brad Wade; Christopher Lloyd; Walther Ellis; Linda Powers

doi:10.1016/S0956-5663(03)00008-3

Reagentless detection of microorganisms by intrinsic fluorescence

Cory Estes
, Andrew Duncan
, Brad Wade
, Christopher Lloyd
, Walther Ellis
, Linda Powers

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels (∼20 cells/cm² or cm³) of microbes in seconds.

Original language	English (US)
Pages (from-to)	511-519
Number of pages	9
Journal	Biosensors and Bioelectronics
Volume	18
Issue number	5-6
DOIs	https://doi.org/10.1016/S0956-5663(03)00008-3
State	Published - May 1 2003

Keywords

Intrinsic fluorescence
Microbe sensor
Microbial contamination
Optical detection

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/S0956-5663(03)00008-3

Cite this

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title = "Reagentless detection of microorganisms by intrinsic fluorescence",

abstract = "Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels (∼20 cells/cm2 or cm3) of microbes in seconds.",

keywords = "Intrinsic fluorescence, Microbe sensor, Microbial contamination, Optical detection",

author = "Cory Estes and Andrew Duncan and Brad Wade and Christopher Lloyd and Walther Ellis and Linda Powers",

note = "Funding Information: This work was supported in part by DARPA under the Biosurveillance Program contract MDA972-97-1-12 and by the Willard L. Eccles Charitable Foundation. ",

year = "2003",

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TY - JOUR

T1 - Reagentless detection of microorganisms by intrinsic fluorescence

AU - Estes, Cory

AU - Duncan, Andrew

AU - Wade, Brad

AU - Lloyd, Christopher

AU - Ellis, Walther

AU - Powers, Linda

N1 - Funding Information: This work was supported in part by DARPA under the Biosurveillance Program contract MDA972-97-1-12 and by the Willard L. Eccles Charitable Foundation.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels (∼20 cells/cm2 or cm3) of microbes in seconds.

AB - Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels (∼20 cells/cm2 or cm3) of microbes in seconds.

KW - Intrinsic fluorescence

KW - Microbe sensor

KW - Microbial contamination

KW - Optical detection

UR - https://www.scopus.com/pages/publications/0037403261

UR - https://www.scopus.com/pages/publications/0037403261#tab=citedBy

U2 - 10.1016/S0956-5663(03)00008-3

DO - 10.1016/S0956-5663(03)00008-3

M3 - Article

C2 - 12706557

AN - SCOPUS:0037403261

SN - 0956-5663

VL - 18

SP - 511

EP - 519

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5-6

ER -

Reagentless detection of microorganisms by intrinsic fluorescence

Abstract

Keywords

ASJC Scopus subject areas

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