Re-sequencing of a virulent strain of Campylobacter jejuni NCTC11168 reveals potential virulence factors

Kerry K. Cooper; Margarethe A. Cooper; Andrea Zuccolo; Lynn A. Joens

doi:10.1016/j.resmic.2012.10.002

Re-sequencing of a virulent strain of Campylobacter jejuni NCTC11168 reveals potential virulence factors

Kerry K. Cooper, Margarethe A. Cooper, Andrea Zuccolo, Lynn A. Joens

Animal and Biomedical Sciences, Research

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In vitro passage of Campylobacter jejuni strains results in phenotypic changes and a general loss of virulence, as is the case with the genome-sequenced strain C. jejuni NCTC11168. Re-sequencing of a virulent strain of NCTC11168 identified 41 SNPs or indels involving 20 genes, four intergenic regions and three pseudogenes. The genes include six motility genes, two chemotaxis genes, three hypothetical genes and a capsule biosynthesis gene, which might have a critical role in C. jejuni virulence. Additionally, we found an insertion in both Cj0676 and Cj1470c, pseudogenes in avirulent NCTC11168, but functional proteins in virulent NCTC11168.

Original language	English (US)
Pages (from-to)	6-11
Number of pages	6
Journal	Research in Microbiology
Volume	164
Issue number	1
DOIs	https://doi.org/10.1016/j.resmic.2012.10.002
State	Published - Jan 2013

Keywords

Campylobacter jejuni
Genome sequencing
NCTC11168
SNPs
Virulence

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1016/j.resmic.2012.10.002

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abstract = "In vitro passage of Campylobacter jejuni strains results in phenotypic changes and a general loss of virulence, as is the case with the genome-sequenced strain C. jejuni NCTC11168. Re-sequencing of a virulent strain of NCTC11168 identified 41 SNPs or indels involving 20 genes, four intergenic regions and three pseudogenes. The genes include six motility genes, two chemotaxis genes, three hypothetical genes and a capsule biosynthesis gene, which might have a critical role in C. jejuni virulence. Additionally, we found an insertion in both Cj0676 and Cj1470c, pseudogenes in avirulent NCTC11168, but functional proteins in virulent NCTC11168.",

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AU - Cooper, Margarethe A.

AU - Zuccolo, Andrea

AU - Joens, Lynn A.

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AB - In vitro passage of Campylobacter jejuni strains results in phenotypic changes and a general loss of virulence, as is the case with the genome-sequenced strain C. jejuni NCTC11168. Re-sequencing of a virulent strain of NCTC11168 identified 41 SNPs or indels involving 20 genes, four intergenic regions and three pseudogenes. The genes include six motility genes, two chemotaxis genes, three hypothetical genes and a capsule biosynthesis gene, which might have a critical role in C. jejuni virulence. Additionally, we found an insertion in both Cj0676 and Cj1470c, pseudogenes in avirulent NCTC11168, but functional proteins in virulent NCTC11168.

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Re-sequencing of a virulent strain of Campylobacter jejuni NCTC11168 reveals potential virulence factors

Abstract

Keywords

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