Rapid flow cytometric analysis of the cell cycle in intact plant tissues

David W. Galbraith; Kristi R. Harkins; Joyce M. Maddox; Nicola M. Ayres; Dharam P. Sharma; Ebrahim Firoozabady

doi:10.1126/science.220.4601.1049

Rapid flow cytometric analysis of the cell cycle in intact plant tissues

David W. Galbraith, Kristi R. Harkins, Joyce M. Maddox, Nicola M. Ayres, Dharam P. Sharma, Ebrahim Firoozabady

Research output: Contribution to journal › Article › peer-review

1686 Scopus citations

Abstract

Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

Original language	English (US)
Pages (from-to)	1049-1051
Number of pages	3
Journal	Science
Volume	220
Issue number	4601
DOIs	https://doi.org/10.1126/science.220.4601.1049
State	Published - 1983
Externally published	Yes

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title = "Rapid flow cytometric analysis of the cell cycle in intact plant tissues",

abstract = "Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.",

author = "Galbraith, {David W.} and Harkins, {Kristi R.} and Maddox, {Joyce M.} and Ayres, {Nicola M.} and Sharma, {Dharam P.} and Ebrahim Firoozabady",

year = "1983",

doi = "10.1126/science.220.4601.1049",

language = "English (US)",

volume = "220",

pages = "1049--1051",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4601",

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TY - JOUR

T1 - Rapid flow cytometric analysis of the cell cycle in intact plant tissues

AU - Galbraith, David W.

AU - Harkins, Kristi R.

AU - Maddox, Joyce M.

AU - Ayres, Nicola M.

AU - Sharma, Dharam P.

AU - Firoozabady, Ebrahim

PY - 1983

Y1 - 1983

N2 - Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

AB - Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

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DO - 10.1126/science.220.4601.1049

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SP - 1049

EP - 1051

JO - Science

JF - Science

IS - 4601

ER -

Rapid flow cytometric analysis of the cell cycle in intact plant tissues

Abstract

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