@article{40e3f3a3c6664a88bbbe1c282181cd4b,
title = "Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions",
abstract = "Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of carbohydrates fructose, glucose, mannose, and mannan, which are involved in many biological and pathogenic phenomena. The proposed detection strategy relies on reversible reactions between boronic acid surfactants and carbohydrates at the hydrocarbon/water interface leading to a dynamic reconfiguration of the droplet morphology, which alters the angular distribution of the droplet's fluorescent light emission. We exploit this unique chemical-morphological-optical coupling to detect Salmonella enterica, a type of bacteria with a well-known binding affinity for mannose. We further demonstrate an oriented immobilization of antibodies at the droplet interface to permit higher selectivity. Our demonstrations yield a new, inexpensive, robust, and generalizable sensing strategy that can help to facilitate the early detection of foodborne pathogens.",
author = "Lukas Zeininger and Sara Nagelberg and Harvey, {Kent S.} and Suchol Savagatrup and Herbert, {Myles B.} and Kosuke Yoshinaga and Capobianco, {Joseph A.} and Mathias Kolle and Swager, {Timothy M.}",
note = "Funding Information: We are grateful for funding from National Institutes of Health of General Medical Sciences, Grant No. GM095843, the Jameel Water and Food Security program at MIT, and the National Science Foundation DMREF-1533985. L.Z. acknowledges support through a fellowship of the German Research Foundation (DFG) #ZE1121/1-1. S.N. and M.K. were supported in part by the U.S. Army Research Office through the Institute for Soldier Nanotechnologies at MIT, under Cooperative Agreement Number W911NF-18-2-0048. S.S. was supported by an F32 Ruth L. Kirschstein National Research Service Award. K.Y. was supported by a Funai Overseas Scholarship. Collaborative work was supported by the U.S. Department of Agriculture, Agricultural Research Service, under Agreement No. 8072-42000-084. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity employer. Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = may,
day = "22",
doi = "10.1021/acscentsci.9b00059",
language = "English (US)",
volume = "5",
pages = "789--795",
journal = "ACS Central Science",
issn = "2374-7943",
publisher = "American Chemical Society",
number = "5",
}