TY - JOUR
T1 - Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
AU - Sullivan, Timothy J.
AU - Dhar, Arun K.
AU - Cruz-Flores, Roberto
AU - Bodnar, Andrea G.
N1 - Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.
AB - Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.
UR - http://www.scopus.com/inward/record.url?scp=85077041271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077041271&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-56170-y
DO - 10.1038/s41598-019-56170-y
M3 - Article
C2 - 31873150
AN - SCOPUS:85077041271
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 19702
ER -