TY - JOUR
T1 - Rapid and sensitive detection of malaria antigen in human blood with lab-on-a-chip
AU - Stemple, C. Christopher
AU - Kwon, Hyuck Jin
AU - Yoon, Jeong Yeol
N1 - Funding Information:
Manuscript received December 26, 2011; accepted May 31, 2012. Date of publication June 20, 2012; date of current version July 24, 2012. This work was supported in part by the Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA), Korea, under Grant C-AD14-2006-11-0. The associate editor coordinating the review of this paper and approving it for publication was Prof. Paul C.-P. Chao.
PY - 2012
Y1 - 2012
N2 - A novel lab-on-a-chip detection device, based on the properties of immunoagglutination, was modified to detect malaria in the human blood through histidine-rich protein 2, an antigen expressed only by Plasmodium falciparum. Utilizing Mie scattering detection, which is angle- and size-dependent, the extent of immunoagglutination could be accurately measured while the optical disturbance from the human blood was minimized. The presence of human serum albumin in the blood is believed to further stabilize the antibody-conjugated submicron beads and/or break off the larger agglutinated beads. The lowest detection limit was 1 pg/mL in 10% of whole blood (equivalent to 10 pg/mL in undiluted whole blood), a few orders of magnitude lower than other assays. The final device is compact, with a fast assay time of approximately 8 min.
AB - A novel lab-on-a-chip detection device, based on the properties of immunoagglutination, was modified to detect malaria in the human blood through histidine-rich protein 2, an antigen expressed only by Plasmodium falciparum. Utilizing Mie scattering detection, which is angle- and size-dependent, the extent of immunoagglutination could be accurately measured while the optical disturbance from the human blood was minimized. The presence of human serum albumin in the blood is believed to further stabilize the antibody-conjugated submicron beads and/or break off the larger agglutinated beads. The lowest detection limit was 1 pg/mL in 10% of whole blood (equivalent to 10 pg/mL in undiluted whole blood), a few orders of magnitude lower than other assays. The final device is compact, with a fast assay time of approximately 8 min.
KW - Latex immunoagglutination
KW - Mie scatter
KW - microfluidics
KW - plasmodium falciparum
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U2 - 10.1109/JSEN.2012.2205072
DO - 10.1109/JSEN.2012.2205072
M3 - Article
AN - SCOPUS:84864546423
SN - 1530-437X
VL - 12
SP - 2735
EP - 2736
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 9
M1 - 6221942
ER -