RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors

Babette Simon; Frank Wattler; Juanita L. Merchant; Karin Münch; Hans Joachim Schütze; Guntram Suske; Rudolf Arnold

doi:10.1016/S0014-5793(97)00736-9

RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors

Babette Simon, Frank Wattler, Juanita L. Merchant, Karin Münch, Hans Joachim Schütze, Guntram Suske, Rudolf Arnold

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1-and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.

Original language	English (US)
Pages (from-to)	383-388
Number of pages	6
Journal	FEBS Letters
Volume	411
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(97)00736-9
State	Published - Jul 14 1997
Externally published	Yes

Keywords

Gastrin promoter
Islet cell
RAP1
Sp-related transcription factor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00736-9

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title = "RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors",

abstract = "Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1-and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.",

keywords = "Gastrin promoter, Islet cell, RAP1, Sp-related transcription factor",

author = "Babette Simon and Frank Wattler and Merchant, {Juanita L.} and Karin M{\"u}nch and Sch{\"u}tze, {Hans Joachim} and Guntram Suske and Rudolf Arnold",

note = "Funding Information: This work was supported by a grant from the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg, Germany, Grant Si 383/3-1 to BS and from the Public Health Service, USA, Grant DK-45729 to J.L.M. who is an investigator of the Howard Hughes Medical Institute.",

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doi = "10.1016/S0014-5793(97)00736-9",

language = "English (US)",

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pages = "383--388",

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T1 - RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors

AU - Simon, Babette

AU - Wattler, Frank

AU - Merchant, Juanita L.

AU - Münch, Karin

AU - Schütze, Hans Joachim

AU - Suske, Guntram

AU - Arnold, Rudolf

N1 - Funding Information: This work was supported by a grant from the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg, Germany, Grant Si 383/3-1 to BS and from the Public Health Service, USA, Grant DK-45729 to J.L.M. who is an investigator of the Howard Hughes Medical Institute.

PY - 1997/7/14

Y1 - 1997/7/14

N2 - Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1-and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.

AB - Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1-and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.

KW - Gastrin promoter

KW - Islet cell

KW - RAP1

KW - Sp-related transcription factor

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors

Abstract

Keywords

ASJC Scopus subject areas

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