Raman microspectroscopy for skin cancer detection in vitro

Chad A. Lieber, Shovan K. Majumder, Dean Billheimer, Darrel L. Ellis, Anita Mahadevan-Jansen

Research output: Contribution to journalArticlepeer-review

109 Scopus citations


We investigate the potential of near-infrared Raman microspectroscopy to differentiate between normal and malignant skin lesions. Thirty-nine skin tissue samples consisting of normal, basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma from 39 patients were investigated. Raman spectra were recorded at the surface and at 20-μm intervals below the surface for each sample, down to a depth of at least 100μm. Data reduction algorithms based on the nonlinear maximum representation and discrimination feature (MRDF) and discriminant algorithms using sparse multinomial logistic regression (SMLR) were developed for classification of the Raman spectra relative to histopathology. The tissue Raman spectra were classified into pathological states with a maximal overall sensitivity and specificity for disease of 100%. These results indicate the potential of using Raman microspectroscopy for skin cancer detection and provide a clear rationale for future clinical studies.

Original languageEnglish (US)
Article number024013
JournalJournal of biomedical optics
Issue number2
StatePublished - 2008


  • Raman spectroscopy
  • biomedical optics
  • confocal optics
  • tissues

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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