Radioreceptor assay for 1α,25-dihydroxyvitamin D3

Peter F. Brumbaugh; David H. Haussler; Rubin Bressler; Mark R. Haussler

doi:10.1126/science.183.4129.1089

Radioreceptor assay for 1α,25-dihydroxyvitamin D3

Peter F. Brumbaugh
, David H. Haussler
, Rubin Bressler
, Mark R. Haussler

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.

Original language	English (US)
Pages (from-to)	1089-1091
Number of pages	3
Journal	Science
Volume	183
Issue number	4129
DOIs	https://doi.org/10.1126/science.183.4129.1089
State	Published - 1974
Externally published	Yes

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title = "Radioreceptor assay for 1α,25-dihydroxyvitamin D3",

abstract = "A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.",

author = "Brumbaugh, \{Peter F.\} and Haussler, \{David H.\} and Rubin Bressler and Haussler, \{Mark R.\}",

year = "1974",

doi = "10.1126/science.183.4129.1089",

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AU - Brumbaugh, Peter F.

AU - Haussler, David H.

AU - Bressler, Rubin

AU - Haussler, Mark R.

PY - 1974

Y1 - 1974

N2 - A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.

AB - A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.

UR - https://www.scopus.com/pages/publications/0015993114

UR - https://www.scopus.com/pages/publications/0015993114#tab=citedBy

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SP - 1089

EP - 1091

JO - Science

JF - Science

IS - 4129

ER -

Radioreceptor assay for 1α,25-dihydroxyvitamin D3

Abstract

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