Abstract
A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.
Original language | English (US) |
---|---|
Pages (from-to) | 1089-1091 |
Number of pages | 3 |
Journal | Science |
Volume | 183 |
Issue number | 4129 |
DOIs | |
State | Published - 1974 |
Externally published | Yes |
ASJC Scopus subject areas
- General