Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Natalia A. Belikova; Ashley Glumac; Ruslan Rafikov; Jianfei Jiang; Joel S. Greenberger; Valerian E. Kagan; Hülya Bayir

doi:10.1016/j.febslet.2009.10.013

Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Natalia A. Belikova, Ashley Glumac, Ruslan Rafikov, Jianfei Jiang, Joel S. Greenberger, Valerian E. Kagan, Hülya Bayir

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H₂O₂ increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512).

Original language	English (US)
Pages (from-to)	3437-3442
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2009.10.013
State	Published - Nov 3 2009
Externally published	Yes

Keywords

Apoptosis
Irradiation
MnSOD
Preconditioning
ROS

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.10.013

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title = "Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase",

abstract = "Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512).",

keywords = "Apoptosis, Irradiation, MnSOD, Preconditioning, ROS",

author = "Belikova, {Natalia A.} and Ashley Glumac and Ruslan Rafikov and Jianfei Jiang and Greenberger, {Joel S.} and Kagan, {Valerian E.} and H{\"u}lya Bayir",

note = "Funding Information: This work was supported by grants from NIH U19-A1068021-01, NS061817, and HL70755.",

year = "2009",

month = nov,

day = "3",

doi = "10.1016/j.febslet.2009.10.013",

language = "English (US)",

volume = "583",

pages = "3437--3442",

journal = "FEBS Letters",

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number = "21",

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TY - JOUR

T1 - Radioprotection by short-term oxidative preconditioning

T2 - Role of manganese superoxide dismutase

AU - Belikova, Natalia A.

AU - Glumac, Ashley

AU - Rafikov, Ruslan

AU - Jiang, Jianfei

AU - Greenberger, Joel S.

AU - Kagan, Valerian E.

AU - Bayir, Hülya

N1 - Funding Information: This work was supported by grants from NIH U19-A1068021-01, NS061817, and HL70755.

PY - 2009/11/3

Y1 - 2009/11/3

N2 - Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512).

AB - Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512).

KW - Apoptosis

KW - Irradiation

KW - MnSOD

KW - Preconditioning

KW - ROS

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DO - 10.1016/j.febslet.2009.10.013

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C2 - 19822147

AN - SCOPUS:70350307201

SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 21

ER -

Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Abstract

Keywords

ASJC Scopus subject areas

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