Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase

Natalia A. Belikova, Ashley Glumac, Ruslan Rafikov, Jianfei Jiang, Joel S. Greenberger, Valerian E. Kagan, Hülya Bayir

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512).

Original languageEnglish (US)
Pages (from-to)3437-3442
Number of pages6
JournalFEBS Letters
Volume583
Issue number21
DOIs
StatePublished - Nov 3 2009
Externally publishedYes

Keywords

  • Apoptosis
  • Irradiation
  • MnSOD
  • Preconditioning
  • ROS

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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