Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

David R. Cox; Margit Burmeister; E. Roydon Price; Suwon Kim; Richard M. Myers

doi:10.1126/science.2218528

Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

David R. Cox, Margit Burmeister, E. Roydon Price, Suwon Kim, Richard M. Myers

Research output: Contribution to journal › Article › peer-review

614 Scopus citations

Abstract

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

Original language	English (US)
Pages (from-to)	245-250
Number of pages	6
Journal	Science
Volume	250
Issue number	4980
DOIs	https://doi.org/10.1126/science.2218528
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.2218528

Cite this

@article{d845217f89834e1aa99113553c8be198,

title = "Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes",

abstract = "Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.",

author = "Cox, {David R.} and Margit Burmeister and Price, {E. Roydon} and Suwon Kim and Myers, {Richard M.}",

year = "1990",

doi = "10.1126/science.2218528",

language = "English (US)",

volume = "250",

pages = "245--250",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4980",

}

TY - JOUR

T1 - Radiation hybrid mapping

T2 - A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

AU - Cox, David R.

AU - Burmeister, Margit

AU - Price, E. Roydon

AU - Kim, Suwon

AU - Myers, Richard M.

PY - 1990

Y1 - 1990

N2 - Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

AB - Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

UR - http://www.scopus.com/inward/record.url?scp=0025131820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025131820&partnerID=8YFLogxK

U2 - 10.1126/science.2218528

DO - 10.1126/science.2218528

M3 - Article

C2 - 2218528

AN - SCOPUS:0025131820

SN - 0036-8075

VL - 250

SP - 245

EP - 250

JO - Science

JF - Science

IS - 4980

ER -

Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this