Radiation attenuates physiological angiogenesis by differential expression of VEGF, Ang-1, Tie-2 and Ang-2 in rat brain

Won Hee Lee, Hyung Joon Cho, William E. Sonntag, Yong Woo Lee

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The etiology of radiation-induced cerebrovascular rarefaction remains unknown. In the present study, we examined the effect of whole-brain irradiation on endothelial cell (EC) proliferation/apoptosis and expression of various angiogenic factors in rat brain. F344×BN rats received either whole-brain irradiation (a single dose of 10 Gy γ rays) or sham irradiation and were maintained for 4, 8 and 24 h after irradiation. Double immunofluorescence staining was employed to visualize EC proliferation/apoptosis in brain. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), endothelial-specific receptor tyrosine kinase (Tie-2), and Ang-2 in brain were determined by real-time RT-PCR and immunofluorescence staining. A significant reduction in CD31-immunoreactive cells was detected in irradiated rat brains compared with sham-irradiated controls. Whole-brain irradiation significantly suppressed EC proliferation and increased EC apoptosis. In addition, a significant decrease in mRNA and protein expression of VEGF, Ang-1 and Tie-2 was observed in irradiated rat brains. In contrast, whole-brain irradiation significantly upregulated Ang-2 expression in rat brains. The present study provides novel evidence that whole-brain irradiation differentially affects mRNA and protein expression of VEGF, Ang-1, Tie-2 and Ang-2. These changes are closely associated with decreased EC proliferation and increased EC apoptosis in brain.

Original languageEnglish (US)
Pages (from-to)753-760
Number of pages8
JournalRadiation Research
Volume176
Issue number6
DOIs
StatePublished - Dec 2011
Externally publishedYes

ASJC Scopus subject areas

  • Radiation
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Radiation attenuates physiological angiogenesis by differential expression of VEGF, Ang-1, Tie-2 and Ang-2 in rat brain'. Together they form a unique fingerprint.

Cite this