@inproceedings{de650443b8ed482ab52a9ba11bcddbb9,
title = "Quantitative, surface heated, droplet polymerase chain reaction for detecting pathogens",
abstract = "A novel quantitative polymerase chain reaction (qPCR) device was developed based on wire-guided droplet manipulation (WDM) method and subsequent real-time quantification utilizing a smartphone or optical fiber/miniature spectrophotometer. The device was initially tested to amplify GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene and further tested to identify Escherichia coli. The lower limit of detection was ∼2 copies per sample. The device is portable with real-time quantification and provides the assay results quickly (30-cycle amplification for 7-20 min) and accurately. The system is also shock and vibration resistant.",
keywords = "Contact angle, Escherichia coli, Real-time polymerase chain reaction",
author = "Angus, {Scott V.} and Soohee Cho and Harshman, {Dustin K.} and Jeong-Yeol Yoon",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "English (US)",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "1452--1454",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}