TY - JOUR
T1 - Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis
AU - Gordon, George S.D.
AU - Joseph, James
AU - Alcolea, Maria P.
AU - Sawyer, Travis
AU - Williams, Calum
AU - Fitzpatrick, Catherine R.M.
AU - Jones, Philip H.
AU - Di Pietro, Massimiliano
AU - Fitzgerald, Rebecca C.
AU - Wilkinson, Timothy D.
AU - Bohndiek, Sarah E.
N1 - Funding Information:
This work was funded by Cancer Research UK (Nos. C47594/ A16267, C14303/A17197, and C47594/A21102); the European Union Seventh Framework Agreement No. FP7-PEOPLE-2013-CIG-630729); and the Pump-Priming Awards from the Cancer Research UK Cambridge Centre, including dedicated funding from the Early Detection Program (No. A20976). We would like to thank Professor Sir Bruce Ponder and Professor Kevin Brindle for early input on our proof-of-concept studies. We would also like to thank summer students Callum Stevens, Sam Watcham, Khoa Pham, and Megan Wilson for their contributions to the tissue phantom characterization instruments that were used as reference gold standards in this work. Data associated with this publication is available at https://doi.org/ 10.17863/CAM.46316.
Publisher Copyright:
© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Phase and polarization of coherent light are highly perturbed by interaction with microstructural changes in premalignant tissue, holding promise for label-free detection of early tumors in endoscopically accessible tissues such as the gastrointestinal tract. Flexible optical multicore fiber (MCF) bundles used in conventional diagnostic endoscopy and endomicroscopy scramble phase and polarization, restricting clinicians instead to low-contrast amplitude-only imaging. We apply a transmission matrix characterization approach to produce full-field en-face images of amplitude, quantitative phase, and resolved polarimetric properties through an MCF. We first demonstrate imaging and quantification of biologically relevant amounts of optical scattering and birefringence in tissue-mimicking phantoms. We present an entropy metric that enables imaging of phase heterogeneity, indicative of disordered tissue microstructure associated with early tumors. Finally, we demonstrate that the spatial distribution of phase and polarization information enables label-free visualization of early tumors in esophageal mouse tissues, which are not identifiable using conventional amplitude-only information.
AB - Phase and polarization of coherent light are highly perturbed by interaction with microstructural changes in premalignant tissue, holding promise for label-free detection of early tumors in endoscopically accessible tissues such as the gastrointestinal tract. Flexible optical multicore fiber (MCF) bundles used in conventional diagnostic endoscopy and endomicroscopy scramble phase and polarization, restricting clinicians instead to low-contrast amplitude-only imaging. We apply a transmission matrix characterization approach to produce full-field en-face images of amplitude, quantitative phase, and resolved polarimetric properties through an MCF. We first demonstrate imaging and quantification of biologically relevant amounts of optical scattering and birefringence in tissue-mimicking phantoms. We present an entropy metric that enables imaging of phase heterogeneity, indicative of disordered tissue microstructure associated with early tumors. Finally, we demonstrate that the spatial distribution of phase and polarization information enables label-free visualization of early tumors in esophageal mouse tissues, which are not identifiable using conventional amplitude-only information.
KW - cancer
KW - optical fibers
KW - polarimetry
KW - quantitative phase imaging
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U2 - 10.1117/1.JBO.24.12.126004
DO - 10.1117/1.JBO.24.12.126004
M3 - Article
C2 - 31840442
AN - SCOPUS:85076541877
VL - 24
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 12
M1 - 126004
ER -