Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals

Thomas W. Vickroy; William R. Roeske; Donald R. Gehlert; James K. Wamsley; Henry I. Yamamura

doi:10.1016/0006-8993(85)90553-0

Quantitative light microscopic autoradiography of [³H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals

Thomas W. Vickroy
, William R. Roeske
, Donald R. Gehlert
, James K. Wamsley
, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

The distribution of specific [³H]hemicholinium-3 ([³H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[³H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [³H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [³H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.

Original language	English (US)
Pages (from-to)	368-373
Number of pages	6
Journal	Brain Research
Volume	329
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(85)90553-0
State	Published - Mar 11 1985

Keywords

[H]hemicholinium-3
cholinergic nerve terminals
high-affinity choline uptake
light microscopic autoradiography

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(85)90553-0

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Vickroy, T. W., Roeske, W. R., Gehlert, D. R., Wamsley, J. K., & Yamamura, H. I. (1985). Quantitative light microscopic autoradiography of [³H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals. Brain Research, 329(1-2), 368-373. https://doi.org/10.1016/0006-8993(85)90553-0

Quantitative light microscopic autoradiography of [³H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals. / Vickroy, Thomas W.; Roeske, William R.; Gehlert, Donald R. et al.
In: Brain Research, Vol. 329, No. 1-2, 11.03.1985, p. 368-373.

Research output: Contribution to journal › Article › peer-review

Vickroy, TW, Roeske, WR, Gehlert, DR, Wamsley, JK & Yamamura, HI 1985, 'Quantitative light microscopic autoradiography of [³H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals', Brain Research, vol. 329, no. 1-2, pp. 368-373. https://doi.org/10.1016/0006-8993(85)90553-0

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title = "Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals",

abstract = "The distribution of specific [3H]hemicholinium-3 ([3H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[3H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [3H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [3H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.",

keywords = "[H]hemicholinium-3, cholinergic nerve terminals, high-affinity choline uptake, light microscopic autoradiography",

author = "Vickroy, \{Thomas W.\} and Roeske, \{William R.\} and Gehlert, \{Donald R.\} and Wamsley, \{James K.\} and Yamamura, \{Henry I.\}",

note = "Funding Information: This work was supportedin part by grantsf romthe Departmenot f Defense( DAMD 17-83C-3023)t,h e American Heart Association Arizona Affiliate (962365), United States Public Health Service Awards (MH 27257a nd MH 30636),a nd Program ProjectG rantHL 20984.T .W.V. is fundedb y a National ResearchS erviceAward from the National Heart, Lung, and Blood Institute. W.R.R. and H.I.Y. are recipientso f a ResearchC areerDevel-opmentA ward (HL-00776)a nd a ResearchS cientist DevelopmenAtw ard (MH-00095,T ype II), respectively.",

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