Abstract
The distribution of specific [3H]hemicholinium-3 ([3H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[3H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [3H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [3H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.
Original language | English (US) |
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Pages (from-to) | 368-373 |
Number of pages | 6 |
Journal | Brain Research |
Volume | 329 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 11 1985 |
Keywords
- [H]hemicholinium-3
- cholinergic nerve terminals
- high-affinity choline uptake
- light microscopic autoradiography
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology