Quantitative determination of pterins in biological fluids by high-performance liquid chromatography

Baldassare stea; Richard M. Halpern; Barbara C. Halpern; Roberts A. Smith

doi:10.1016/S0021-9673(00)81259-5

Quantitative determination of pterins in biological fluids by high-performance liquid chromatography

Baldassare stea
, Richard M. Halpern
, Barbara C. Halpern
, Roberts A. Smith

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.

Original language	English (US)
Pages (from-to)	363-375
Number of pages	13
Journal	Journal of Chromatography A
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(00)81259-5
State	Published - Feb 1 1980
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)81259-5

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@article{d77577e15a2a43e9a974c0e09f435ba9,

title = "Quantitative determination of pterins in biological fluids by high-performance liquid chromatography",

abstract = "During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.",

author = "Baldassare stea and Halpern, \{Richard M.\} and Halpern, \{Barbara C.\} and Smith, \{Roberts A.\}",

note = "Funding Information: This work was suppated by the Wius and Dorothy Fried Research Foundation and by the Celh\&ara nd Molecukr Biology Training Grant No. GM 07185-W",

year = "1980",

month = feb,

day = "1",

doi = "10.1016/S0021-9673(00)81259-5",

language = "English (US)",

volume = "188",

pages = "363--375",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Quantitative determination of pterins in biological fluids by high-performance liquid chromatography

AU - stea, Baldassare

AU - Halpern, Richard M.

AU - Halpern, Barbara C.

AU - Smith, Roberts A.

N1 - Funding Information: This work was suppated by the Wius and Dorothy Fried Research Foundation and by the Celh&ara nd Molecukr Biology Training Grant No. GM 07185-W

PY - 1980/2/1

Y1 - 1980/2/1

N2 - During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.

AB - During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.

UR - https://www.scopus.com/pages/publications/0018886130

UR - https://www.scopus.com/pages/publications/0018886130#tab=citedBy

U2 - 10.1016/S0021-9673(00)81259-5

DO - 10.1016/S0021-9673(00)81259-5

M3 - Article

C2 - 7380932

AN - SCOPUS:0018886130

SN - 0021-9673

VL - 188

SP - 363

EP - 375

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Quantitative determination of pterins in biological fluids by high-performance liquid chromatography

Abstract

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