Quantitative determination of pterins in biological fluids by high-performance liquid chromatography

Baldassare stea, Richard M. Halpern, Barbara C. Halpern, Roberts A. Smith

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.

Original languageEnglish (US)
Pages (from-to)363-375
Number of pages13
JournalJournal of Chromatography A
Issue number2
StatePublished - Feb 1 1980

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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