TY - JOUR
T1 - Quantitative determination of pterins in biological fluids by high-performance liquid chromatography
AU - stea, Baldassare
AU - Halpern, Richard M.
AU - Halpern, Barbara C.
AU - Smith, Roberts A.
N1 - Funding Information:
This work was suppated by the Wius and Dorothy Fried Research Foundation and by the Celh&ara nd Molecukr Biology Training Grant No. GM 07185-W
PY - 1980/2/1
Y1 - 1980/2/1
N2 - During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.
AB - During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.
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U2 - 10.1016/S0021-9673(00)81259-5
DO - 10.1016/S0021-9673(00)81259-5
M3 - Article
C2 - 7380932
AN - SCOPUS:0018886130
SN - 0021-9673
VL - 188
SP - 363
EP - 375
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -