TY - GEN
T1 - Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles
AU - Stamm, M. T.
AU - Trickey-Glassman, A. S.
AU - Jiang, L.
AU - Zohar, Y.
PY - 2013
Y1 - 2013
N2 - Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumor cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalized particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. While particle desorption and cellular loss of binding affinity in time seem to be negligible, cell-particle-cell interaction was identified as the limiting mechanism for the number of particles bound to target cells. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.
AB - Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumor cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalized particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. While particle desorption and cellular loss of binding affinity in time seem to be negligible, cell-particle-cell interaction was identified as the limiting mechanism for the number of particles bound to target cells. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.
KW - functionalized particles
KW - microfluidics
KW - particle-cell binding
KW - receptor-ligand interaction
KW - specific targeting
UR - http://www.scopus.com/inward/record.url?scp=84883093941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883093941&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2013.6559916
DO - 10.1109/NEMS.2013.6559916
M3 - Conference contribution
AN - SCOPUS:84883093941
SN - 9781467363525
T3 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
SP - 1108
EP - 1111
BT - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
T2 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Y2 - 7 April 2013 through 10 April 2013
ER -