TY - JOUR
T1 - Quantification of riboflavin, riboflavin 5′-phosphate and flavin adenine dinucleotide in plasma and urine by high-performance liquid chromatography
AU - Lopez-Anaya, Arturo
AU - Mayersohn, Michael
N1 - Funding Information:
This work was supported by a grant from the National Institute on Aging (AG03460). The authors gratefully acknowledge the competent technical assistance of Robyn Kessler. Support of one of the authors (A.L.-A.) was provided by The National Autonomous University of Mexico.
PY - 1987
Y1 - 1987
N2 - A high-performance liquid chromatographic method with fluorimetric detection for the quantification of riboflavin (RB), riboflavin 5′-phosphate (FMN), and flavin adenine dinucleotide (FAD) in plasma, whole blood, and urine is described. Under isocratic conditions with a reversed-phase column, the compounds are completely resolved and eluted within 9 min. Plasma proteins are precipitated with acetonitrile followed by shaking the aqueous phase with chloroform. Urine samples are diluted and injected directly. The reproducibility of this method for the quantification of RB in plasma has a between-day coefficient of variation of 6%. The application of this method is illustrated by analyzing plasma and urine samples from a human subject who received an intravenous dose of FMN equivalent to 25 mg of RB.
AB - A high-performance liquid chromatographic method with fluorimetric detection for the quantification of riboflavin (RB), riboflavin 5′-phosphate (FMN), and flavin adenine dinucleotide (FAD) in plasma, whole blood, and urine is described. Under isocratic conditions with a reversed-phase column, the compounds are completely resolved and eluted within 9 min. Plasma proteins are precipitated with acetonitrile followed by shaking the aqueous phase with chloroform. Urine samples are diluted and injected directly. The reproducibility of this method for the quantification of RB in plasma has a between-day coefficient of variation of 6%. The application of this method is illustrated by analyzing plasma and urine samples from a human subject who received an intravenous dose of FMN equivalent to 25 mg of RB.
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U2 - 10.1016/0378-4347(87)80332-8
DO - 10.1016/0378-4347(87)80332-8
M3 - Article
C2 - 3443641
AN - SCOPUS:0023567381
SN - 0378-4347
VL - 423
SP - 105
EP - 113
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -