Quantification of proprofol in small volumes of plasma using high-performance liquid chromatography with fluorescence detection. Therapeutic drug monitoring of one patient during and after infusion in the perioperative period of cardiac surgery

A liquid chromatographic method with fluorescence detection for determination of propofol in small volumes of plasma has been adapted to the conditions of the laboratory using a C18 column. Peaks were eluted at 21 min propofol and at 12 min thymol (internal standard) using a binary mobile phase at flow rate 0.8 mL/min. The method validated presents 0.05 mg/L sensitivity, 0.1-10 mg/L linearity, (r² 0.9977), systematic error of 8.2 and 7.0% (intra- and interday accuracy), intra- and interday precision (CV %: 8.4% and 8.8%). Recovery 93.9%, good stability and robustness were reported. The analytical method was validated by the quantification of propofol in arterial plasma (C_max:3.3 mg/L C^min: 0.2mg/L) of one patient received 1570 mg infusion of propofol in the perioperative period of surgery of myocardial revascularization. Depth of anaesthesia expressed as bispectral index (BIS) was 36 (32-40) in that period up to the 4^th h, and an adequate hypnosis was obtained until the end of surgery.