Abstract
A liquid chromatographic method with fluorescence detection for determination of propofol in small volumes of plasma has been adapted to the conditions of the laboratory using a C18 column. Peaks were eluted at 21 min propofol and at 12 min thymol (internal standard) using a binary mobile phase at flow rate 0.8 mL/min. The method validated presents 0.05 mg/L sensitivity, 0.1-10 mg/L linearity, (r2 0.9977), systematic error of 8.2 and 7.0% (intra- and interday accuracy), intra- and interday precision (CV %: 8.4% and 8.8%). Recovery 93.9%, good stability and robustness were reported. The analytical method was validated by the quantification of propofol in arterial plasma (Cmax:3.3 mg/L Cmin: 0.2mg/L) of one patient received 1570 mg infusion of propofol in the perioperative period of surgery of myocardial revascularization. Depth of anaesthesia expressed as bispectral index (BIS) was 36 (32-40) in that period up to the 4th h, and an adequate hypnosis was obtained until the end of surgery.
Original language | English (US) |
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Pages (from-to) | 748-754 |
Number of pages | 7 |
Journal | Latin American Journal of Pharmacy |
Volume | 26 |
Issue number | 5 |
State | Published - Sep 2007 |
Externally published | Yes |
Keywords
- CLAE-F
- Heart surgery
- Propofol
- Therapeutic drug monitoring
ASJC Scopus subject areas
- Pharmaceutical Science