Quantification of proprofol in small volumes of plasma using high-performance liquid chromatography with fluorescence detection. Therapeutic drug monitoring of one patient during and after infusion in the perioperative period of cardiac surgery

Carlos R. Da Silva Filho, Roberto T.O. Kondo, Andréia Z. Chignalia, Karin V. López, Valeria A. Pereira, Maria J.C. Carmona, José O.C. Auler, Silvia R.C.J. Santos

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A liquid chromatographic method with fluorescence detection for determination of propofol in small volumes of plasma has been adapted to the conditions of the laboratory using a C18 column. Peaks were eluted at 21 min propofol and at 12 min thymol (internal standard) using a binary mobile phase at flow rate 0.8 mL/min. The method validated presents 0.05 mg/L sensitivity, 0.1-10 mg/L linearity, (r2 0.9977), systematic error of 8.2 and 7.0% (intra- and interday accuracy), intra- and interday precision (CV %: 8.4% and 8.8%). Recovery 93.9%, good stability and robustness were reported. The analytical method was validated by the quantification of propofol in arterial plasma (Cmax:3.3 mg/L Cmin: 0.2mg/L) of one patient received 1570 mg infusion of propofol in the perioperative period of surgery of myocardial revascularization. Depth of anaesthesia expressed as bispectral index (BIS) was 36 (32-40) in that period up to the 4th h, and an adequate hypnosis was obtained until the end of surgery.

Original languageEnglish (US)
Pages (from-to)748-754
Number of pages7
JournalLatin American Journal of Pharmacy
Volume26
Issue number5
StatePublished - Sep 2007
Externally publishedYes

Keywords

  • CLAE-F
  • Heart surgery
  • Propofol
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

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